	US 12,104,193 B2
	Method for enzymatic sulfurylation of alcohols and amines using bacterium of the family Enterobacteriaceae
Sergey Vasilievich Smirnov, Moscow (RU); and Irina Lvovna Tokmakova, Moscow (RU)
Assigned to AJINOMOTO CO., INC., Tokyo (JP)
Filed by AJINOMOTO CO., INC., Tokyo (JP)
Filed on Dec. 23, 2020, as Appl. No. 17/132,270.
Application 17/132,270 is a continuation of application No. PCT/JP2019/028570, filed on Jul. 11, 2019.
Prior Publication US 2021/0115484 A1, Apr. 22, 2021
Int. Cl. C12P 19/26 (2006.01); C12N 9/10 (2006.01); C12N 9/16 (2006.01); C12N 15/52 (2006.01)

CPC C12P 19/26 (2013.01) [C12N 9/13 (2013.01); C12N 9/16 (2013.01); C12N 15/52 (2013.01); C12Y 208/02001 (2013.01); C12Y 208/02008 (2013.01); C12Y 301/03002 (2013.01); C12Y 301/03007 (2013.01)]

17 Claims

1. A method for enzymatic sulfurylation of a substrate or for producing a sulfated derivative of a substrate comprising:

(i) reacting the substrate with 3′-phosphoadenosine-5′-phosphosulfate in a medium containing a bacterium belonging to the genus Escherichia to produce a sulfated derivative of said substrate, and

(ii) collecting the sulfated derivative from the medium;

wherein said substrate has a chemical group selected from the group consisting of a hydroxyl group, an amino group, and combinations thereof; and

wherein said bacterium has been modified

(A) to produce, at least, a protein having sulfotransferase activity, and

(B) to attenuate expression of an aphA gene or a cysQ gene.