US 12,104,168 B2
Cell culture medium
Iwona Szczerbinska, Singapore (SG); Yun Shen Chan, Singapore (SG); and Huck Hui Ng, Singapore (SG)
Assigned to AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH (A*STAR), Singapore (SG)
Appl. No. 17/054,108
Filed by Agency For Science, Technology And Research, Singapore (SG)
PCT Filed May 10, 2019, PCT No. PCT/SG2019/050265
§ 371(c)(1), (2) Date Nov. 9, 2020,
PCT Pub. No. WO2019/216831, PCT Pub. Date Nov. 14, 2019.
Claims priority of application No. 10201803944U (SG), filed on May 10, 2018.
Prior Publication US 2021/0371810 A1, Dec. 2, 2021
Int. Cl. C12N 5/00 (2006.01); C12N 5/071 (2010.01)
CPC C12N 5/0018 (2013.01) [C12N 5/0672 (2013.01); C12N 5/0678 (2013.01); C12N 5/068 (2013.01); C12N 2501/01 (2013.01); C12N 2501/105 (2013.01); C12N 2501/11 (2013.01); C12N 2501/12 (2013.01); C12N 2501/13 (2013.01); C12N 2501/135 (2013.01); C12N 2501/16 (2013.01); C12N 2501/165 (2013.01); C12N 2501/727 (2013.01)] 8 Claims
 
1. A method for preparing a stem cell from a differentiated cell from the gut lineage, the method comprising contacting a differentiated cell from the gut lineage with a cell culture medium comprising: a) a base medium; b) an ABL and SRC dual kinase inhibitor, or an ABL kinase inhibitor and a SRC kinase inhibitor; c) a mitogenic factor; and d) a WNT signalling pathway activator, for a time and under conditions to derive a stem cell from the differentiated cell.