	US 12,104,150 B2
	Near-infrared photothermally activated CRISPR/CAS9 genome editing machine
Hanyong Peng, Edmonton (CA); Hongquan Zhang, Ottawa (CA); Xing-Fang Li, Edmonton (CA); and Xiaochun Le, Edmonton (CA)
Assigned to The Governors of the University of Alberta, Edmonton (CA)
Filed by The Governors of the University of Alberta, Edmonton (CA)
Filed on Sep. 14, 2020, as Appl. No. 17/020,676.
Claims priority of provisional application 62/899,927, filed on Sep. 13, 2019.
Prior Publication US 2021/0079373 A1, Mar. 18, 2021
Int. Cl. C12N 15/10 (2006.01); C12N 9/22 (2006.01); C12N 13/00 (2006.01); C12N 15/113 (2010.01)

CPC C12N 15/102 (2013.01) [C12N 9/22 (2013.01); C12N 13/00 (2013.01); C12N 15/113 (2013.01); C12N 2310/3519 (2013.01)]

16 Claims

1. An isolated protector polynucleotide for reversible binding to an sgRNA, consisting of or comprising the structure of Formula (I),

T-S-L (I)

wherein,

T comprises a targeting sequencing domain polynucleotide that reversibly hybridizes to a target binding domain of an sgRNA;

S comprises a stem domain polynucleotide sequence that forms a hairpin structure when said protector polynucleotide is not bound to said sgRNA; and

L comprises a linker polynucleotide sequence having a first end attached to said S, and a second end for attachment to a nanostructure,

wherein the protector polynucleotide and the sgRNA are separate nucleic acid molecules and wherein said T is displaceable from said sgRNA upon exposure of the isolated protector polynucleotide to a low-power NIR laser.