US 12,104,139 B2
Processes for obtaining microbial oil from microbial cells
Neil Francis Leininger, Winchester, KY (US); Ginger Shank, Winchester, KY (US); Xiao Dong, Woodstock, MD (US); Joseph William Pfeifer, III, Westminster, MD (US); and Vidya Pai, Columbia, MD (US)
Assigned to DSM IP Assets B.V., (NL)
Filed by DSM IP ASSETS B.V., TE Heerlen (NL)
Filed on Sep. 6, 2019, as Appl. No. 16/563,260.
Application 16/563,260 is a continuation of application No. 15/106,379, abandoned, previously published as PCT/US2014/071469, filed on Dec. 19, 2014.
Claims priority of provisional application 62/093,986, filed on Dec. 18, 2014.
Claims priority of provisional application 61/919,000, filed on Dec. 20, 2013.
Prior Publication US 2019/0390135 A1, Dec. 26, 2019
Int. Cl. C11B 1/02 (2006.01); C07C 51/42 (2006.01); C07C 51/487 (2006.01); C11B 3/00 (2006.01)
CPC C11B 1/02 (2013.01) [C07C 51/42 (2013.01); C07C 51/487 (2013.01); C11B 1/025 (2013.01); C11B 3/001 (2013.01)] 47 Claims
 
1. A process for obtaining a microbial oil comprising one or more polyunsaturated fatty acids from one or more microbial cells, wherein the process comprises:
(a) lysing the cells comprising the microbial oil to form a lysed cell composition;
(b) treating the lysed cell composition to form an oil-containing emulsion;
(c) separating the oil-containing emulsion from the lysed cell composition;
(d) demulsifying the oil-containing emulsion, the demulsifying comprising adding an acid comprising citric acid and further comprising adding a base; and
(e) recovering the oil.