	US 12,103,949 B2
	Protein recovery
Abhiram Arunkumar, Watertown, MA (US); Nripen Singh, Acton, MA (US); and Ameya Umesh Borwankar, San Francisco, CA (US)
Assigned to Bristol-Myers Squibb Company, Princeton, NJ (US)
Appl. No. 17/267,873
Filed by BRISTOL-MYERS SQUIBB COMPANY, Princeton, NJ (US)
PCT Filed Aug. 13, 2019, PCT No. PCT/US2019/046250 § 371(c)(1), (2) Date Feb. 11, 2021, PCT Pub. No. WO2020/036903, PCT Pub. Date Feb. 20, 2020.
Claims priority of provisional application 62/718,864, filed on Aug. 14, 2018.
Prior Publication US 2021/0171573 A1, Jun. 10, 2021
Int. Cl. C07K 16/00 (2006.01); C07K 1/34 (2006.01); C07K 16/24 (2006.01); C07K 16/28 (2006.01)

CPC C07K 1/34 (2013.01) [C07K 16/244 (2013.01); C07K 16/2803 (2013.01); C07K 16/2866 (2013.01); C07K 16/2878 (2013.01); C07K 16/2896 (2013.01); C07K 2319/30 (2013.01); C07K 2319/31 (2013.01)]

35 Claims

1. A method of reducing or preventing dilution of a target protein during a recovery flush after a filtration comprising (i) passing a sample containing the target protein through a filtration assembly at a feed flow rate while the filtration is on and (ii) passing a buffer through the filtration assembly during a recovery flush at a flush flow rate that is between about 10 and about 40 liters per square meter per hour (LMH), thereby reducing or preventing dilution of the target protein compared to the dilution of a target protein obtained with a flush flow rate of 300 LMH, wherein the Reynold's number (“Re”) of the flow in the recovery flush is between about 1 and about 50, wherein the Re is calculated with the following formula:

Re=Dυρ/μ, and

wherein D is the diameter of the channel (m) or equivalent diameter in the case of non-cylindrical flow channel geometries, υ is average velocity (m/s) (Q/A_c), ρ is density (kg/m³), and μ is the viscosity (Pa·s).