	US 12,103,004 B2
	Method and apparatus for processing tissue and other samples encoding cellular spatial position information
Stevan Bogdan Jovanovich, Livermore, CA (US)
Assigned to SILICON VALLEY SCIENTIFIC, INC., Livermore, CA (US)
Appl. No. 16/980,358
Filed by SILICON VALLEY SCIENTIFIC, INC., Livermore, CA (US)
PCT Filed Mar. 12, 2019, PCT No. PCT/US2019/021942 § 371(c)(1), (2) Date Sep. 11, 2020, PCT Pub. No. WO2019/178164, PCT Pub. Date Sep. 19, 2019.
Claims priority of provisional application 62/670,234, filed on May 11, 2018.
Claims priority of provisional application 62/641,425, filed on Mar. 12, 2018.
Prior Publication US 2021/0046482 A1, Feb. 18, 2021
Int. Cl. B01L 3/00 (2006.01); C12N 15/10 (2006.01); G01N 1/28 (2006.01); G01N 35/00 (2006.01); G01N 35/08 (2006.01); G16B 5/00 (2019.01)

CPC B01L 3/502784 (2013.01) [B01L 3/502761 (2013.01); B01L 3/50857 (2013.01); C12N 15/1013 (2013.01); G01N 1/286 (2013.01); G01N 35/0099 (2013.01); G01N 35/08 (2013.01); G16B 5/00 (2019.02); B01L 2200/025 (2013.01); B01L 2200/026 (2013.01); B01L 2200/027 (2013.01); B01L 2200/028 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/0896 (2013.01); B01L 2300/18 (2013.01)]

22 Claims

1. A method comprising:

a) providing a frozen biological tissue specimen on a sample carrier, wherein cells in the tissue sample have a spatial position in the tissue specimen, and wherein the sample carrier comprises (i) a perforated support that supports the tissue specimen and (ii) an array of passages at addressable positions through the sample carrier and positioned below the perforated support;

b) disrupting the tissue specimen to release one or a plurality of cells or nuclei; and

c) collecting a microsample comprising the one or a plurality of released cells or nuclei through the perforations into the passages, wherein the addressable position of a passage indicates the original spatial position of the one or a plurality of cells or nuclei moved into the passage.