a cover, a container body, a sealing portion, a driving portion and a liquid guide portion, the liquid guide portion having at least a liquid inlet portion, a cleaning liquid inlet portion, and a liquid outlet portion;

wherein the cover is detachably and fixedly connected to the container body, and the cover is provided with a ventilation structure and a bacteria-retaining sealing breathable structure for making the internal and external air pressure of the bioreactor consistent;

wherein the liquid inlet penetrates the sidewall of the container body to deliver the liquid to be replaced into the container body;

wherein the cleaning liquid inlet portion includes a cleaning liquid inlet pipe that penetrates the bottom of the driving portion to communicate with the inside of the driving portion, so as to deliver cleaning liquid into the driving portion;

wherein the liquid outlet portion penetrates the sidewall and the bottom of the container body to discharge the replacement liquid or the cleaned liquid out of the bioreactor;

wherein a gas-liquid channel is opened at the bottom of the container body;

wherein the driving portion is used to drive the replacement liquid or the cleaning liquid to move up and down through elastic deformation; and

wherein the sealing portion is arranged along the outer sidewall of the bioreactor, and is used for fixedly connecting the container body and the driving portion to prevent the liquid from flowing out of the sidewall of the bioreactor.