| CPC A01H 1/08 (2013.01) [A01H 1/021 (2021.01); A01H 1/023 (2021.01); A01H 5/06 (2013.01); A01H 5/10 (2013.01); A01H 6/20 (2018.05); C07K 14/415 (2013.01); C12N 15/8287 (2013.01)] | 11 Claims |
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1. A plant having a biological activity of a haploid inducer and comprising a polynucleotide which comprises a nucleotide sequence encoding a centromeric histone H3 (CENH3) protein, wherein the nucleotide sequence comprises at least one introduced mutation that alters the amino acid sequence of the CENH3 protein in an N-terminal domain and confers the biological activity of a haploid inducer to the CENH3 protein,
wherein
i. the amino acid arginine at position 3 of SEQ ID NO: 1 is substituted for lysine,
ii. the amino acid arginine at position 3 of SEQ ID NO: 20 is substituted for glutamine,
iii. the amino acid arginine at position 10 of SEQ ID NO: 1 is substituted for phenylalanine,
iv. the amino acid serine at position 9 of SEQ ID NO: 14 is substituted for phenylalanine,
v. the amino acid arginine at position 16 of SEQ ID NO: 14 is substituted for glutamine,
vi. the amino acid proline at position 16 of SEQ ID NO: 20 is substituted for leucine,
vii. the amino acid serine at position 24 of SEQ ID NO: 14 is substituted for leucine,
viii. the amino acid alanine at position 25 of SEQ ID NO: 17 is substituted for threonine,
ix. the amino acid glutamic acid at position 29 of SEQ ID NO: 14 is substituted for lysine,
x. the amino acid glycine at position 30 of SEQ ID NO: 14 is substituted for aspartic acid,
xi. the amino acid alanine at position 33 of SEQ ID NO: 14 or at position 32 of SEQ ID NO: 20 is substituted for threonine,
xii. the amino acid proline at position 35 of SEQ ID NO: 14 is substituted for leucine,
xiii. the amino acid glutamic acid at position 35 of SEQ ID NO: 20 is substituted for lysine,
xiv. the amino acid serine at position 41 of SEQ ID NO: 14 is substituted for asparagine,
xv. the amino acid glycine at position 42 of SEQ ID NO: 17 is substituted for glutamic acid,
xvi. the amino acid glycine at position 43 of SEQ ID NO: 14 is substituted for glutamic acid,
xvii. the amino acid proline at position 50 of SEQ ID NO: 14 is substituted for serine,
xviii. the amino acid proline at position 55 of SEQ ID NO: 14 is substituted for leucine,
xix. the amino acid glutamic acid at position 55 of SEQ ID NO: 17 is substituted for lysine,
xx. the amino acid glycine at position 57 of SEQ ID NO: 14 is substituted for aspartic acid,
xxi. the amino acid glycine at position 61 of SEQ ID NO: 14 is substituted for glutamic acid,
xxii. the amino acid arginine at position 65 of SEQ ID NO: 14 is substituted for glutamine,
xxiii. the amino acid arginine at position 65 of SEQ ID NO: 14 is substituted for stop signal,
xxiv. the amino acid proline at position 71 of SEQ ID NO: 14 is substituted for serine,
xxv. the amino acid aspartic acid at position 46 of SEQ ID NO: 23 is substituted for asparagine or glycine,
xxvi. the amino acid lysine at position 7 of SEQ ID NO: 2 is substituted for serine,
xxvii. the amino acid proline at position 56 of SEQ ID NO: 20 is substituted for serine,
xxviii. the amino acid proline at position 14 of SEQ ID NO: 2 is substituted for valine, or
xxix. the amino acid alanine at position 62 of SEQ ID NO: 17 is substituted for valine.
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