	US 11,774,454 B2
	Non-invasive analysis of embryo metabolites
Marcello Rubessa, Champaign, IL (US); Matthew B. Wheeler, Tolono, IL (US); Andrea Ambrosi, Urbana, IL (US); Scott E. Denmark, Champaign, IL (US); and Dianelys Gonzalez-Pena, Kalamazoo, MI (US)
Assigned to The Board of Trustees of the University of Illinois, Urbana, IL (US)
Filed by THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, Urbana, IL (US)
Filed on May 12, 2023, as Appl. No. 17/1,094.
Claims priority of provisional application 62/891,362, filed on Aug. 25, 2019.
Prior Publication US 2021/0055303 A1, Feb. 25, 2021
Int. Cl. G01N 33/68 (2006.01); G01N 33/50 (2006.01); G01N 33/483 (2006.01); A01K 45/00 (2006.01)

CPC G01N 33/6812 (2013.01) [A01K 45/00 (2013.01); G01N 33/4833 (2013.01); G01N 33/5091 (2013.01)]

11 Claims

1. A method for determining the gender of an embryo, the method comprising:

(i) growing an embryo in an in vitro culture media; and

(ii) identifying the amount of valine, pyruvate, or both valine and pyruvate contained in the spent culture media during early development, late development, or a combination thereof; wherein the presence of valine during early development in a concentration above a baseline indicates that the embryo will more likely be male than female, and the presence of pyruvate during late development in a concentration above a baseline indicates that the embryo will more likely be male than female; and

(iii) selecting an embryo based on a likely gender and implanting the embryo into a uterus.