US 11,774,450 B2
Apparatus for detection of proteolytic activity in a biological sample
Shubhendra Singh Thakur, Nagpur (IN); and Deovrat Begde, Nagpur (IN)
Assigned to Shubhendra Singh Thakur, Nagpur (IN); Deovrat Begde, Nagpur (IN); and CURO Biosciences Pvt. Ltd., Nagpur (IN)
Filed by Shubhendra Singh Thakur, Nagpur (IN); Deovrat Begde, Nagpur (IN); and CURO Biosciences Pvt. Ltd., Nagpur (IN)
Filed on Jan. 21, 2021, as Appl. No. 17/153,896.
Claims priority of application No. 202021002695 (IN), filed on Jan. 21, 2020.
Prior Publication US 2021/0223245 A1, Jul. 22, 2021
Int. Cl. G01N 33/573 (2006.01); G01N 33/574 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/573 (2013.01) [G01N 33/574 (2013.01); G01N 33/582 (2013.01); C12Y 304/17 (2013.01)] 14 Claims
OG exemplary drawing
 
1. An apparatus (100) for the detection of a proteolytic activity level of a biological sample, the apparatus (100) consisting of:
two or more inner segment tubes (101, 103, 105) stacked one on top of the other; a cross-sectional thin auxiliary layer (108), consisting of a staining agent, at an upper opening of at least one of the inner segment tubes (101, 103, 105); a tubular outer jacket (109) with a seal cap (107), wherein the inner segment tubes (101, 103, 105) are stacked in the tubular outer jacket (109); and a cross-sectional protein substrate layer (102, 104, 106) having an individual thickness of at least 1 mm within each of the inner segment tubes (101, 103, 105), wherein the apparatus (100) detects the proteolytic activity level of the biological sample based on the number of protein substrate layers (102, 104, 106) digested by the biological sample, as the biological sample passes from a first inner segment tube (101, 103) to a next inner segment tube (103, 105).