US 11,774,444 B2
Detection and identification of cell bound and soluble antigens using magnetic levitation
Ionita C. Ghiran, Winchester, MA (US); Nathan Shapiro, Groton, MA (US); Anish V. Sharda, Brookline, MA (US); and Edward J. Felton, Medfield, MA (US)
Assigned to Beth Israel Deaconess Medical Center, Inc., Boston, MA (US)
Appl. No. 16/349,235
Filed by Beth Israel Deaconess Medical Center, Inc., Boston, MA (US)
PCT Filed Nov. 10, 2017, PCT No. PCT/US2017/061048
§ 371(c)(1), (2) Date May 10, 2019,
PCT Pub. No. WO2018/089760, PCT Pub. Date May 17, 2018.
Claims priority of provisional application 62/421,151, filed on Nov. 11, 2016.
Prior Publication US 2019/0293639 A1, Sep. 26, 2019
Int. Cl. G01N 33/543 (2006.01); B03C 1/01 (2006.01); B03C 1/033 (2006.01); B03C 1/30 (2006.01); B03C 1/32 (2006.01); G01N 9/36 (2006.01); G01N 33/50 (2006.01); G01N 35/00 (2006.01); G01N 33/569 (2006.01); G01N 33/537 (2006.01)
CPC G01N 33/54326 (2013.01) [B03C 1/01 (2013.01); B03C 1/0332 (2013.01); B03C 1/30 (2013.01); B03C 1/32 (2013.01); G01N 9/36 (2013.01); G01N 33/5005 (2013.01); G01N 33/5044 (2013.01); G01N 33/537 (2013.01); G01N 33/56966 (2013.01); G01N 33/56972 (2013.01); G01N 35/0098 (2013.01); B03C 2201/26 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A detection device for detecting and identifying an antigen in a biological sample using magnetic levitation, the device comprising:
a magnetic levitation apparatus including:
a pair of magnets configured to generate a magnetic field;
a container holder arranged between the pair of magnets;
a container containing a solution comprising antibody-coated beads configured to attach to target cell-bound antigens in the biological sample when the biological sample is added to the solution in the container, the solution and the biological sample forming a mixture, wherein, when the container is positioned in the container holder such that the mixture is exposed to the magnetic field generated by the pair of magnets, bead-cell complexes formed between the antibody-coated beads and the target cell-bound antigens in the biological sample magnetically levitate within the container; and
an imaging device coupled to the magnetic levitation apparatus and configured to capture at least one image of the bead-cell complexes in the mixture and to identify the target cell-bound antigen when the mixture is present in the magnetic field of the magnetic levitation apparatus.
 
17. A portable point of care (POC) device configured to perform magnetic levitation to detect a presence of an antigen, the portable POC device comprising:
a magnetic levitation apparatus including:
a pair of magnets configured to generate a magnetic field,
a container holder arranged between the pair of magnets, and
a container containing a solution comprising antibody-coated beads,
wherein:
the antibody-coated beads are configured to attach to target cell-bound antigens in a biological sample added to the solution, the solution and the biological sample forming a mixture, and
when the container is positioned in the container holder such that the mixture is exposed to the magnetic field generated by the pair of magnets, bead-cell complexes formed between the antibody-coated beads and the target cell-bound antigens in the biological sample magnetically levitate within the container;
an imaging device coupled to the magnetic levitation apparatus and configured to capture at least one image of the bead-cell complexes in the mixture and to identify the target cell-bound antigens, when the mixture is present in the magnetic field of the magnetic levitation apparatus; and
a display configured to display information about the target cell-bound antigens in the mixture determined based, at least in part, on the at least one image captured by the imaging device.