US 11,774,366 B2
Sequencing nucleic acids via surface enhanced Raman spectroscopy
Gemma Mendonsa, Minneapolis, MN (US); Eric K. Wadleigh, Shakopee, MN (US); Vivek Krishnamurthy, Normandale, MN (US); Riyan A. Mendonsa, Minneapolis, MN (US); Martin G. Blaber, Normandale, MN (US); and Krishnan Subramanian, Shakopee, MN (US)
Assigned to SEAGATE TECHNOLOGY LLC, Fremont, CA (US)
Filed by Seagate Technology LLC, Fremont, CA (US)
Filed on Aug. 5, 2020, as Appl. No. 16/986,108.
Claims priority of provisional application 62/938,264, filed on Nov. 20, 2019.
Prior Publication US 2021/0148828 A1, May 20, 2021
Int. Cl. C12Q 1/6869 (2018.01); G01N 21/65 (2006.01); C12N 9/12 (2006.01); G01N 27/447 (2006.01); B82Y 30/00 (2011.01)
CPC G01N 21/658 (2013.01) [C12N 9/1252 (2013.01); C12Q 1/6869 (2013.01); G01N 27/44786 (2013.01); B82Y 30/00 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of sequencing a DNA strand, comprising:
passing the DNA strand through a nanochannel hot spot of a Raman sensor bounded by plasmonic nanostructures and excited by at least one laser;
identifying the nucleotides of a first section of the DNA strand present in the nanochannel at a first period in time by a Raman signature, and identifying the nucleotides of a second section of the DNA strand present in the nanochannel at a second period in time by a second Raman signature; and
comparing the identified nucleotides of the first section to the identified nucleotides of the second section to identify a change.