	US 11,774,341 B2
	Quantification of nanobubble reactivity in water
Paul K. Westerhoff, Scottsdale, AZ (US); Shahnawaz Sinha, Chandler, AZ (US); and Yuqiang Bi, Chandler, AZ (US)
Assigned to Arizona Board of Regents on behalf of Arizona State University, Scottsdale, AZ (US)
Filed by Paul K. Westerhoff, Scottsdale, AZ (US); Shahnawaz Sinha, Chandler, AZ (US); and Yuqiang Bi, Chandler, AZ (US)
Filed on Sep. 9, 2021, as Appl. No. 17/447,259.
Claims priority of provisional application 63/075,969, filed on Sep. 9, 2020.
Prior Publication US 2022/0074841 A1, Mar. 10, 2022
Int. Cl. G01N 15/06 (2006.01); G01N 31/22 (2006.01); G01N 15/00 (2006.01)

CPC G01N 15/06 (2013.01) [G01N 31/22 (2013.01); G01N 2015/0011 (2013.01); G01N 2015/0681 (2013.01); G01N 2015/0693 (2013.01)]

17 Claims

1. A method of quantifying nanobubbles in solution, the method comprising:

combining an indicator with a fluid comprising nanobubbles to yield a first solution;

bursting the nanobubbles in the first solution to yield a second solution; and

assessing a difference between the first solution and the second solution to yield:

a concentration of the nanobubbles in the first solution,

a concentration of reactive oxygen species in the first solution or the second solution, or both,

wherein the indicator comprises methylene blue, and the reactive oxygen species oxidizes the methylene blue, thus producing a colon metric reaction between the methylene and the reactive oxygen species, and

wherein the colorimetric reaction produces color depletion of methylene blue, which is related back to the concentration of nanobubbles in the second solution.