	US 11,773,439 B2
	Nucleic acid sequencing-by-synthesis (SBS) methods that combine SBS cycle steps
Eli N. Glezer, Del Mar, CA (US); Andrew Spaventa, La Jolla, CA (US); and David Barker, Del Mar, CA (US)
Assigned to Singular Genomics Systems, Inc., San Diego, CA (US)
Filed by Singular Genomics Systems, Inc., San Diego, CA (US)
Filed on Nov. 4, 2022, as Appl. No. 18/52,798.
Application 18/052,798 is a continuation of application No. 16/492,076, granted, now 11,591,647, previously published as PCT/US2018/021219, filed on Mar. 6, 2018.
Claims priority of provisional application 62/467,751, filed on Mar. 6, 2017.
Prior Publication US 2023/0111634 A1, Apr. 13, 2023
Int. Cl. C12Q 1/6869 (2018.01); C07H 21/04 (2006.01); C12Q 1/6867 (2018.01)

CPC C12Q 1/6869 (2013.01) [C07H 21/04 (2013.01); C12Q 1/6867 (2013.01); C12Q 2521/101 (2013.01); C12Q 2525/185 (2013.01); C12Q 2525/186 (2013.01); C12Q 2535/00 (2013.01)]

20 Claims

1. A method of sequencing a DNA molecule, comprising:

(a) contacting a solid support comprising a primed template DNA molecule with a deoxyribonucleotide triphosphate (dNTP) analogue species, wherein said dNTP analogue species is complementary to a nucleotide of the primed template DNA molecule, and wherein said dNTP analogue species is associated with a detectable label;

(b) after step (a), contacting said primed template DNA molecule with a plurality of blocked dNTP analogue species, and incorporating with a polymerase at least one of said plurality of blocked dNTP analogue species, thereby forming a blocked extension product; and

(c) imaging said solid support during steps (a) and (b), wherein the combined steps (a)-(b) occurs for about 1 minute to about 10 minutes.