US 11,773,421 B2
Method for producing fructose-6-phosphate from dihydroxy acetone phosphate and glyceraldehyde-3-phosphate
Alfredo Alarcon Yanez, Grenoble (FR); Samia Boudah, Vincennes (FR); and Romain Chayot, Paris (FR)
Assigned to Global Bioenergies, Evry (FR)
Appl. No. 17/263,401
Filed by Global Bioenergies, Evry (FR)
PCT Filed Jul. 26, 2019, PCT No. PCT/EP2019/070147
§ 371(c)(1), (2) Date Jan. 26, 2021,
PCT Pub. No. WO2020/021051, PCT Pub. Date Jan. 30, 2020.
Claims priority of application No. 18186082 (EP), filed on Jul. 27, 2018.
Prior Publication US 2022/0112532 A1, Apr. 14, 2022
Int. Cl. C12P 19/02 (2006.01); C12N 9/16 (2006.01); C12N 9/88 (2006.01); C12N 9/90 (2006.01)
CPC C12P 19/02 (2013.01) [C12N 9/16 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12Y 202/01002 (2013.01); C12Y 301/03001 (2013.01); C12Y 301/03002 (2013.01); C12Y 301/03021 (2013.01); C12Y 301/03023 (2013.01); C12Y 301/03038 (2013.01); C12Y 301/03074 (2013.01); C12Y 401/02 (2013.01); C12Y 504/02002 (2013.01); C12Y 504/02008 (2013.01)] 18 Claims
 
1. A method for the production of fructose-6-phosphate (F6P) from dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)
(A) comprising the steps of:
(a) enzymatically converting dihydroxyacetone phosphate (DHAP) into dihydroxyacetone (DHA) using a phosphoric monoester hydrolase (EC 3.1.3.-); and
(b) enzymatically converting the thus produced dihydroxyacetone (DHA) together with glyceraldehyde-3-phosphate (G3P) into fructose-6-phosphate (F6P) using an aldehyde lyase (EC 4.1.2.-) or a transaldolase (EC 2.2.1.2); or
(B) comprising the steps of:
(a′) enzymatically converting glyceraldehyde-3-phosphate (G3P) into glyceraldehyde using a phosphoric monoester hydrolase (EC 3.1.3.-); and
(b′) enzymatically converting the thus produced glyceraldehyde together with dihydroxyacetone phosphate (DHAP) into fructose-1-phosphate (F1P) using a fructose bisphosphate aldolase (EC 4.1.2.13); and
(c′) enzymatically converting the thus produced fructose-1-phosphate (F1P) into fructose-6-phosphate (F6P) using a phosphoglucomutase (EC 5.4.2.2) or a phosphomannomutase (EC 5.4.2.8).