CPC C12P 19/02 (2013.01) [C12N 9/16 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12Y 202/01002 (2013.01); C12Y 301/03001 (2013.01); C12Y 301/03002 (2013.01); C12Y 301/03021 (2013.01); C12Y 301/03023 (2013.01); C12Y 301/03038 (2013.01); C12Y 301/03074 (2013.01); C12Y 401/02 (2013.01); C12Y 504/02002 (2013.01); C12Y 504/02008 (2013.01)] | 18 Claims |
1. A method for the production of fructose-6-phosphate (F6P) from dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)
(A) comprising the steps of:
(a) enzymatically converting dihydroxyacetone phosphate (DHAP) into dihydroxyacetone (DHA) using a phosphoric monoester hydrolase (EC 3.1.3.-); and
(b) enzymatically converting the thus produced dihydroxyacetone (DHA) together with glyceraldehyde-3-phosphate (G3P) into fructose-6-phosphate (F6P) using an aldehyde lyase (EC 4.1.2.-) or a transaldolase (EC 2.2.1.2); or
(B) comprising the steps of:
(a′) enzymatically converting glyceraldehyde-3-phosphate (G3P) into glyceraldehyde using a phosphoric monoester hydrolase (EC 3.1.3.-); and
(b′) enzymatically converting the thus produced glyceraldehyde together with dihydroxyacetone phosphate (DHAP) into fructose-1-phosphate (F1P) using a fructose bisphosphate aldolase (EC 4.1.2.13); and
(c′) enzymatically converting the thus produced fructose-1-phosphate (F1P) into fructose-6-phosphate (F6P) using a phosphoglucomutase (EC 5.4.2.2) or a phosphomannomutase (EC 5.4.2.8).
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