US 11,773,420 B2
Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
Bertus Noordam, Echt (NL); Loes Elizabeth Bevers, Echt (NL); Rudy Francois Maria Jozef Parton, Echt (NL); and Michael Petrus Jozef Berkhout, Echt (NL)
Assigned to Versalis S.p.A., San Donato (IT)
Filed by VERSALIS S.P.A., San Donato Milanese (IT)
Filed on Feb. 8, 2021, as Appl. No. 17/170,169.
Application 17/170,169 is a continuation of application No. 16/408,062, filed on May 9, 2019, granted, now 10,947,573.
Application 16/408,062 is a continuation of application No. 15/307,241, granted, now 10,337,040, issued on Jul. 2, 2019, previously published as PCT/EP2015/059316, filed on Apr. 29, 2015.
Claims priority of application No. 14166538 (EP), filed on Apr. 30, 2014; application No. 14166539 (EP), filed on Apr. 30, 2014; application No. 14166545 (EP), filed on Apr. 30, 2014; application No. 14167284 (EP), filed on May 7, 2014; and application No. 14167483 (EP), filed on May 8, 2014.
Prior Publication US 2021/0180098 A1, Jun. 17, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/02 (2006.01); C13K 1/02 (2006.01); D21C 3/26 (2006.01); C13K 13/00 (2006.01); C12P 19/14 (2006.01)
CPC C12P 19/02 (2013.01) [C12P 19/14 (2013.01); C13K 1/02 (2013.01); C13K 13/00 (2013.01); C13K 13/002 (2013.01); D21C 3/26 (2013.01); C12P 2203/00 (2013.01); Y02E 50/10 (2013.01)] 19 Claims
 
1. A process for the preparation of a sugar product from lignocellulosic material, comprising:
a) optionally, pretreatment of the lignocellulosic material,
b) optionally, washing of the optionally pretreated lignocellulosic material,
c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises a lytic polysaccharide monooxygenase (LPMO), and
d) optionally, recovery of a glucose-containing composition,
wherein the amount of formed gluconic acid at the end of the enzymatic hydrolysis by oxidation by LPMO of the lignocellulosic material containing cellulose and/or cello-oligosaccharides is kept between 3 to 10 g/kg glucan present in the lignocellulosic material by adding a suitable amount of oxygen during the enzymatic hydrolysis to the lignocellulosic material.