	US 11,773,389 B2
	Single cell analysis of transposase accessible chromatin
Zahra Kamila Belhocine, Fremont, CA (US); Geoffrey McDermott, Livermore, CA (US); Francesca Meschi, Pleasanton, CA (US); and Xinying Zheng, San Jose, CA (US)
Assigned to 10x Genomics, Inc., Pleasanton, CA (US)
Filed by 10X GENOMICS, INC., Pleasanton, CA (US)
Filed on Oct. 1, 2021, as Appl. No. 17/492,536.
Application 17/492,536 is a continuation of application No. 16/841,411, filed on Apr. 6, 2020, granted, now 11,155,810.
Application 16/841,411 is a continuation of application No. 16/419,555, filed on May 22, 2019, abandoned.
Application 16/419,555 is a continuation in part of application No. 16/206,168, filed on Nov. 30, 2018, granted, now 11,198,866.
Application 16/206,168 is a continuation in part of application No. PCT/US2018/034774, filed on May 25, 2018.
Application 16/206,168 is a continuation of application No. 15/842,687, filed on Dec. 14, 2017, granted, now 10,400,235, issued on Sep. 30, 2019.
Claims priority of provisional application 62/650,223, filed on Mar. 29, 2018.
Claims priority of provisional application 62/527,529, filed on Jun. 30, 2017.
Claims priority of provisional application 62/511,905, filed on May 26, 2017.
Prior Publication US 2022/0259586 A1, Aug. 18, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/10 (2006.01)

CPC C12N 15/1065 (2013.01) [C12N 15/1068 (2013.01); C12N 15/1075 (2013.01); C12N 15/1096 (2013.01)]

22 Claims

1. A method of processing a sample, comprising:

(a) contacting a plurality of cells or cell nuclei comprising chromatin with a plurality of transposase nucleic acid complexes to generate a cell or cell nucleus comprising a tagged fragment of genomic deoxyribonucleic acid (DNA);

(b) partitioning said plurality of cells or cell nuclei and a plurality of barcode sequences into a plurality of partitions, wherein a partition of said plurality of partitions comprises:

(i) said cell or cell nucleus comprising said tagged fragment of genomic DNA;

(ii) a first barcode oligonucleotide molecule comprising a first barcode sequence;

(iii) a second barcode oligonucleotide molecule comprising a second barcode sequence; and

(iv) a reverse transcriptase;

(c) using said first barcode oligonucleotide molecule and said tagged fragment of genomic DNA to generate a first barcoded molecule comprising (1) a sequence of said tagged fragment of genomic DNA, and (2) said first barcode sequence, or a reverse complement thereof; and

(d) using said second barcode oligonucleotide molecule, said reverse transcriptase, and a ribonucleic acid (RNA) molecule of said cell or cell nucleus to generate a second barcoded molecule comprising (1) a complementary DNA (cDNA) sequence of said RNA molecule and (2) said second barcode sequence, or a reverse complement thereof.