US 11,773,365 B2
Cell concentration methods and devices for use in automated bioreactors
Joseph O'Connor, Walkersville, MD (US); Erika McAfee, Walkersville, MD (US); Samatha Bandapalle, Walkersville, MD (US); Yaling Shi, Walkersville, MD (US); and Eytan Abraham, Walkersville, MD (US)
Assigned to LONZA WALKERSVILLE, INC., Walkersville, MD (US)
Filed by LONZA WALKERSVILLE, INC., Walkersville, MD (US)
Filed on Feb. 5, 2020, as Appl. No. 16/782,181.
Claims priority of provisional application 62/803,219, filed on Feb. 8, 2019.
Prior Publication US 2020/0255793 A1, Aug. 13, 2020
Int. Cl. C12M 1/00 (2006.01); B01D 61/14 (2006.01); C12M 1/36 (2006.01); B01L 3/00 (2006.01); C12M 1/34 (2006.01); B01D 71/52 (2006.01); B01D 71/34 (2006.01); B01D 71/42 (2006.01)
CPC C12M 47/02 (2013.01) [B01D 61/145 (2013.01); B01D 71/34 (2013.01); B01D 71/42 (2013.01); B01D 71/52 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); C12M 29/04 (2013.01); C12M 41/26 (2013.01); C12M 41/30 (2013.01); C12M 41/48 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A cassette for use in an automated cell engineering system, comprising:
(a) a cell culture chamber;
(b) a pumping system fluidly connected to the cell culture chamber;
(c) a tangential flow filter fluidly connected to the pumping system, wherein the pumping system provides a retentate flow to the tangential flow filter and wherein a permeate flow of the tangential flow filter is controlled by a flow controller; and
(d) a cellular sample output fluidly connected to the tangential flow filter,
wherein (a)-(d) are self-contained and removable, and the cassette comprises a cell culture, a culture media, a cell wash media, an activation reagent, and/or a vector,
wherein the tangential flow filter is at an angle of about 3° to about 20° relative to the horizontal such that an exit end of the tangential flow filter is disposed vertically above an input end of the tangential flow filter, and
wherein the cassette is contained within an enclosable housing of the automated cell engineering system.