US 11,773,142 B2
Recombinant adenoviruses and uses thereof
Dan H. Barouch, Newton, MA (US); Peter Abbink, Winthrop, MA (US); Mark Justin Iampietro, Boston, MA (US); and Menzo J. E. Havenga, Boston, MA (US)
Assigned to Beth Israel Deaconess Medical Center, Inc., Boston, MA (US)
Appl. No. 16/772,045
Filed by Beth Israel Deaconess Medical Center, Inc., Boston, MA (US)
PCT Filed Dec. 11, 2018, PCT No. PCT/US2018/064978
§ 371(c)(1), (2) Date Jun. 11, 2020,
PCT Pub. No. WO2019/118480, PCT Pub. Date Jun. 20, 2019.
Claims priority of provisional application 62/641,774, filed on Mar. 12, 2018.
Claims priority of provisional application 62/607,288, filed on Dec. 18, 2017.
Claims priority of provisional application 62/597,268, filed on Dec. 11, 2017.
Prior Publication US 2020/0399323 A1, Dec. 24, 2020
Int. Cl. C07K 14/075 (2006.01); A61K 35/761 (2015.01); A61K 39/235 (2006.01); A61K 31/7088 (2006.01); C07K 14/54 (2006.01); C12N 15/861 (2006.01)
CPC C07K 14/075 (2013.01) [A61K 31/7088 (2013.01); A61K 35/761 (2013.01); A61K 39/235 (2013.01); C07K 14/5425 (2013.01); C12N 15/861 (2013.01)] 26 Claims
 
1. A recombinant adenovirus comprising:
(I) a nucleotide sequence encoding a hexon protein, wherein:
(a) the nucleotide sequence encoding the hexon protein has at least 93% sequence identity over the entire sequence of SEQ ID NO: 56; or
(b) the hexon protein comprises 99% or greater sequence identity over the entire amino acid sequence of SEQ ID NO: 160;
(II) a nucleotide sequence encoding a short fiber protein, a long fiber protein, and/or a penton protein, wherein:
(a) the nucleotide sequence encoding the short fiber protein has at least 85% identity to the nucleotide sequence of SEQ ID NO: 19;
(b) the short fiber protein has at least 85% identity to the amino acid sequence of SEQ ID NO: 123;
(c) the nucleotide sequence encoding the long fiber protein has at least 90% identity to the nucleotide sequence of SEQ ID NO: 42;
(d) the long fiber protein has at least 90% identity to the amino acid sequence of SEQ ID NO: 146;
(e) the nucleotide sequence encoding the penton protein has at least 95% sequence identity over the entire sequence of SEQ ID NO: 108, and/or
(f) the penton protein comprises 96% or greater sequence identity over the entire amino acid sequence of SEQ ID NO: 212; and
(III) a deletion in or of the E1, E2, and/or E3 region.