US 11,772,061 B2
Methods of fabricating hyper compliant polymer particles and methods of use and compositions
Eric M. Darling, Norfolk, MA (US); Nicholas R. Labriola, Farmington, NY (US); and Edith Mathiowitz, Brookline, MA (US)
Assigned to Brown University, Providence, RI (US)
Appl. No. 16/465,374
Filed by Brown University, Providence, RI (US)
PCT Filed Nov. 30, 2017, PCT No. PCT/US2017/063825
§ 371(c)(1), (2) Date May 30, 2019,
PCT Pub. No. WO2018/102480, PCT Pub. Date Jun. 7, 2018.
Claims priority of provisional application 62/520,066, filed on Jun. 15, 2017.
Claims priority of provisional application 62/428,029, filed on Nov. 30, 2016.
Prior Publication US 2019/0329210 A1, Oct. 31, 2019
Int. Cl. A61K 9/50 (2006.01); A61K 9/51 (2006.01); A61K 9/00 (2006.01); A61K 9/16 (2006.01); B01J 13/14 (2006.01); B01J 13/22 (2006.01); B01L 3/00 (2006.01)
CPC B01J 13/14 (2013.01) [A61K 9/5021 (2013.01); A61K 9/5073 (2013.01); A61K 9/5089 (2013.01); A61K 9/5138 (2013.01); B01J 13/22 (2013.01); B01L 3/50273 (2013.01); B01L 2200/148 (2013.01)] 5 Claims
 
1. Cell mimicking microparticles (CMMPs) consisting of the following:
1. hyper compliant polymer particles (HCPPs) consisting of synthetic materials cross-linked with a cross-linker and having a mechanical compliance from 0.01 kPa to less than 10 kPa, the synthetic materials selected from the group consisting of a polyacrylamide, a poly(N-vinyl formamide), a polyethylene oxide, a dendrimer, a star polymer, a bioerodible polymer, a polydimethyl siloxane (PDMS), and a combination thereof;
wherein the HCPPs comprise a predetermined size with a monodisperse diameter within a range of about 5 μm to about 40 μm, and including such that the HCPPs are each operative to encapsulate an agent; and,
2. a coating on each of the HCPPs, with said coating including at least one material selected from the group consisting of a protein, a nucleotide sequence, a carbohydrate, a lipid, a cell plasma membrane, a therapeutic agent, a dye targeting agent, and a combination thereof;
wherein the coating extends to the entirety of each of the HCPPs, and each HCPP is thereby entirely encased by coating.