US 11,772,017 B2
Separation of cells based on size and affinity using paper microfluidic device
Charles R. Mace, Watertown, MA (US); Syrena C. Fernandes, Somerville, MA (US); and Samuel B. Berry, Orangeburg, NY (US)
Assigned to Trustee of Tufts College, Medford, MA (US)
Filed by Trustee of Tufts College, Medford, MA (US)
Filed on Aug. 28, 2020, as Appl. No. 16/948,066.
Application 16/948,066 is a division of application No. 16/033,261, filed on Jul. 12, 2018, granted, now 10,758,846.
Application 16/033,261 is a continuation in part of application No. PCT/US2017/013065, filed on Jan. 11, 2017.
Claims priority of provisional application 62/277,810, filed on Jan. 12, 2016.
Prior Publication US 2020/0391139 A1, Dec. 17, 2020
Int. Cl. B01D 29/01 (2006.01); B01D 29/58 (2006.01); B01D 39/18 (2006.01); G01N 33/49 (2006.01); G01N 33/574 (2006.01); G01N 31/22 (2006.01)
CPC B01D 29/01 (2013.01) [B01D 29/58 (2013.01); B01D 39/18 (2013.01); G01N 33/491 (2013.01); G01N 33/57415 (2013.01); G01N 31/22 (2013.01)] 27 Claims
OG exemplary drawing
 
21. A method for providing a microfluidic device, the method comprising: providing a first layer of a porous material with pores having a first average pore size, the first layer having a liquid-receiving area through which a liquid sample is received into the microfluidic device; stacking below the first layer a distinct second layer of another porous material having pores of a second average pore size; and positioning a channel within the second layer, the channel having a starting end positioned at least in part in an overlapping manner with the liquid-receiving area of the first layer, the channel having a terminating end extending laterally at a predetermined wicking distance from the starting end; wherein, the first average pore size and the second average pore size are selected such that, upon the receiving of the liquid sample, a wicking effect is caused in which at least some of the liquid sample flows along the channel at least a portion of the wicking distance between the starting end and the terminating end.