	US 11,771,804 B2
	Bone grafts including osteogenic stem cells, and methods relating to the same
Archana Bhat, Phoenixville, PA (US); Shairali Rao, Austin, TX (US); and Daniel Laskowitz, Lancaster, PA (US)
Assigned to Globus Medical, Inc., Audubon, PA (US)
Filed by GLOBUS MEDICAL, INC., Audubon, PA (US)
Filed on Aug. 18, 2021, as Appl. No. 17/405,113.
Application 15/362,889 is a division of application No. 14/057,806, filed on Oct. 18, 2013, granted, now 9,539,286, issued on Jan. 10, 2017.
Application 17/405,113 is a continuation of application No. 16/009,832, filed on Jun. 15, 2018, granted, now 11,116,874.
Application 16/009,832 is a continuation of application No. 15/362,889, filed on Nov. 29, 2016, granted, now 10,022,474, issued on Jul. 17, 2018.
Prior Publication US 2021/0369919 A1, Dec. 2, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A61L 27/38 (2006.01); A61K 35/28 (2015.01); A61L 27/36 (2006.01); A61K 35/32 (2015.01); C12N 5/077 (2010.01); C12N 5/0775 (2010.01); A61K 35/12 (2015.01)

CPC A61L 27/3834 (2013.01) [A61K 35/28 (2013.01); A61K 35/32 (2013.01); A61L 27/365 (2013.01); A61L 27/3608 (2013.01); A61L 27/3687 (2013.01); A61L 27/3847 (2013.01); C12N 5/0654 (2013.01); C12N 5/0663 (2013.01); A61K 2035/124 (2013.01); A61L 2430/02 (2013.01); C12N 2533/14 (2013.01); C12N 2533/54 (2013.01)]

18 Claims

1. A composition having viable cells comprising:

a putty-like bone graft stored in a freezing media comprising DMSO under freezing condition, the putty-like bone graft comprising osteogenic stem cells and a scaffold, the scaffold comprising a mixture of cortico-cancellous chips and demineralized cortical bone, the cortico-cancellous chips containing bone material from both cancellous bone and cortical bone contained in condyles and only the condyles excluding the cortical shaft, the demineralized cortical bone containing bone material in cortical shafts of long bones, wherein the osteogenic stem cells are viable cells capable of bone regeneration.