US 11,771,803 B2
Enhancement of osteogenic potential of bone grafts
Jill Helms, Stanford, CA (US); and Girija Dhamdhere, San Jose, CA (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed on Jan. 15, 2020, as Appl. No. 16/743,837.
Application 16/743,837 is a continuation of application No. 15/868,395, filed on Jan. 11, 2018, granted, now 10,568,989.
Application 15/868,395 is a continuation of application No. 15/063,317, filed on Mar. 7, 2016, granted, now 9,925,304, issued on Mar. 27, 2018.
Application 15/063,317 is a continuation of application No. 14/333,220, filed on Jul. 16, 2014, granted, now 9,301,980, issued on Apr. 5, 2016.
Claims priority of provisional application 61/957,946, filed on Jul. 16, 2013.
Prior Publication US 2020/0147267 A1, May 14, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 9/127 (2006.01); A61K 35/28 (2015.01); C12N 5/0775 (2010.01); A61L 27/36 (2006.01); A61K 38/17 (2006.01); A61K 35/12 (2015.01)
CPC A61L 27/3687 (2013.01) [A61K 9/127 (2013.01); A61K 35/28 (2013.01); A61K 38/1709 (2013.01); A61L 27/365 (2013.01); C12N 5/0663 (2013.01); A61K 2035/124 (2013.01); A61L 2430/02 (2013.01); C12N 2501/415 (2013.01)] 18 Claims
 
1. A method of repairing a bone, comprising:
(a) obtaining a bone graft material from a subject in need thereof;
(b) contacting the bone graft material ex-vivo with a liposome comprising a Wnt protein to produce a modified human bone graft material; and
(c) administering the modified bone graft material to the bone of the subject, wherein the subject receives a spinal fusion.