CPC A61K 41/00 (2013.01) [A61K 31/7088 (2013.01); A61N 5/062 (2013.01); C07K 14/195 (2013.01); C07K 14/215 (2013.01); C07K 14/37 (2013.01); C07K 2319/60 (2013.01); C12N 2750/14143 (2013.01); C12N 2810/6027 (2013.01)] | 19 Claims |
1. A method to relieve neuropathic pain in a subject in need of such relief, comprising
transfecting dorsal root ganglion (DRG) neurons of the subject with a polynucleotide using an adeno-associated viral (AAV) vector by intrathecal injection, wherein the polynucleotide comprises a regulatory region driving expression of a sequence encoding
archaerhodopsin-3 (Arch) and the regulatory region comprises at least one of a preprotachykinin-A (PPT) promoter, a voltage-gated sodium channel subunit alpha (Scn10a) promoter, and a vanilloid receptor subtype 1 (TRPV1) promoter, and exposing Arch-expressing DRG neurons to light comprising a wavelength of from 495-570 nm, wherein the exposing Arch-expressing DRG neurons to light leads to a decrease in membrane potential relative to DRG neurons not expressing Arch.
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