US 11,771,763 B2
Methods and compositions for decreasing chronic pain
Alan Horsager, Los Angeles, CA (US); Kenneth Greenberg, Oakland, CA (US); Benjamin C. Matteo, San Francisco, CA (US); Edward S. Boyden, Chestnut Hill, MA (US); Douglas G. Ririe, Winston-Salem, NC (US); James C. Eisenach, Winston-Salem, NC (US); and Christian Wentz, Cambridge, MA (US)
Assigned to EOS NEUROSCIENCE, INC., San Francisco, CA (US); and WAKE FOREST UNIVERSITY HEALTH SCIENCES, Winston-Salem, NC (US)
Filed by EOS Neuroscience, Inc., San Francisco, CA (US); and Wake Forest University Health Sciences, Winston-Salem, NC (US)
Filed on Jun. 27, 2019, as Appl. No. 16/455,077.
Application 16/455,077 is a continuation of application No. 13/637,977, abandoned, previously published as PCT/US2011/031297, filed on Apr. 5, 2011.
Claims priority of provisional application 61/321,117, filed on Apr. 5, 2010.
Prior Publication US 2021/0069329 A1, Mar. 11, 2021
Int. Cl. A61N 5/06 (2006.01); A61K 48/00 (2006.01); A61K 49/00 (2006.01); A61K 41/00 (2020.01); C07K 14/195 (2006.01); C07K 14/37 (2006.01); A61K 31/7088 (2006.01); C07K 14/215 (2006.01)
CPC A61K 41/00 (2013.01) [A61K 31/7088 (2013.01); A61N 5/062 (2013.01); C07K 14/195 (2013.01); C07K 14/215 (2013.01); C07K 14/37 (2013.01); C07K 2319/60 (2013.01); C12N 2750/14143 (2013.01); C12N 2810/6027 (2013.01)] 19 Claims
 
1. A method to relieve neuropathic pain in a subject in need of such relief, comprising
transfecting dorsal root ganglion (DRG) neurons of the subject with a polynucleotide using an adeno-associated viral (AAV) vector by intrathecal injection, wherein the polynucleotide comprises a regulatory region driving expression of a sequence encoding
archaerhodopsin-3 (Arch) and the regulatory region comprises at least one of a preprotachykinin-A (PPT) promoter, a voltage-gated sodium channel subunit alpha (Scn10a) promoter, and a vanilloid receptor subtype 1 (TRPV1) promoter, and exposing Arch-expressing DRG neurons to light comprising a wavelength of from 495-570 nm, wherein the exposing Arch-expressing DRG neurons to light leads to a decrease in membrane potential relative to DRG neurons not expressing Arch.