US 11,771,755 B2
Self-asssembling nanostructure vaccines
Neil King, Seattle, WA (US); David Baker, Seattle, WA (US); Lance Stewart, Seattle, WA (US); Brooke Fiala, Seattle, WA (US); Daniel Ellis, Seattle, WA (US); Lauren Carter, Seattle, WA (US); Rashmi Ravichandran, Seattle, WA (US); George Ueda, Seattle, WA (US); Jorge Fallas, Seattle, WA (US); and Una Nattermann, Seattle, WA (US)
Assigned to University of Washington, Seattle, WA (US)
Appl. No. 16/971,278
Filed by UNIVERSITY OF WASHINGTON, Seattle, WA (US)
PCT Filed Feb. 28, 2019, PCT No. PCT/US2019/020029
§ 371(c)(1), (2) Date Aug. 19, 2020,
PCT Pub. No. WO2019/169120, PCT Pub. Date Sep. 6, 2019.
Claims priority of provisional application 62/724,721, filed on Aug. 30, 2018.
Claims priority of provisional application 62/636,757, filed on Feb. 28, 2018.
Prior Publication US 2020/0397886 A1, Dec. 24, 2020
Int. Cl. A61K 39/12 (2006.01); C12N 15/86 (2006.01); B82Y 5/00 (2011.01); B82Y 40/00 (2011.01)
CPC A61K 39/12 (2013.01) [C12N 15/86 (2013.01); B82Y 5/00 (2013.01); B82Y 40/00 (2013.01); C12N 2710/16134 (2013.01); C12N 2710/16234 (2013.01); C12N 2760/00034 (2013.01)] 20 Claims
 
1. A self-assembling icosahedral protein nanostructure, comprising:
20 trimeric assemblies having 3-fold symmetry, each trimeric assembly comprising 3 trimer proteins, each trimer protein comprising a polypeptide consisting of, in N- to C-terminal order, an extracellular domain of influenza HA, a polypeptide linker, and a trimer subunit comprising a polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence selected from SEQ ID NO: 7 or 29-31; and
12 pentameric assemblies having 5-fold symmetry, each pentameric assembly comprising 5 pentamer subunits, wherein each pentamer subunit comprises a polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence selected from SEQ ID NO: 8 or 32-34;
wherein the trimeric assemblies and the pentameric assemblies form an icosahedron.