CPC A61K 39/12 (2013.01) [C12N 15/86 (2013.01); B82Y 5/00 (2013.01); B82Y 40/00 (2013.01); C12N 2710/16134 (2013.01); C12N 2710/16234 (2013.01); C12N 2760/00034 (2013.01)] | 20 Claims |
1. A self-assembling icosahedral protein nanostructure, comprising:
20 trimeric assemblies having 3-fold symmetry, each trimeric assembly comprising 3 trimer proteins, each trimer protein comprising a polypeptide consisting of, in N- to C-terminal order, an extracellular domain of influenza HA, a polypeptide linker, and a trimer subunit comprising a polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence selected from SEQ ID NO: 7 or 29-31; and
12 pentameric assemblies having 5-fold symmetry, each pentameric assembly comprising 5 pentamer subunits, wherein each pentamer subunit comprises a polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence selected from SEQ ID NO: 8 or 32-34;
wherein the trimeric assemblies and the pentameric assemblies form an icosahedron.
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