US 11,771,728 B2
Materials and methods for increasing the tocopherol content in seed oil
Carl Andre, Research Triangle Park, NC (US)
Assigned to BASF PLANT SCIENCE COMPANY GMBH, Ludwigshafen (DE)
Filed by BASF PLANT SCIENCE COMPANY GMBH, Ludwigshafen (DE)
Filed on Nov. 9, 2020, as Appl. No. 17/93,232.
Application 17/093,232 is a continuation of application No. 16/405,440, filed on May 7, 2019, granted, now 10,829,775.
Application 16/405,440 is a continuation of application No. 15/525,768, granted, now 10,760,089, previously published as PCT/EP2015/076608, filed on Nov. 13, 2015.
Claims priority of provisional application 62/234,373, filed on Sep. 29, 2015.
Claims priority of provisional application 62/079,622, filed on Nov. 14, 2014.
Prior Publication US 2021/0054395 A1, Feb. 25, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); A61K 36/31 (2006.01); C12Q 1/6895 (2018.01); C12N 9/02 (2006.01); C12N 9/00 (2006.01); A61K 31/202 (2006.01)
CPC A61K 36/31 (2013.01) [C12Q 1/6895 (2013.01); A61K 31/202 (2013.01); C12N 9/0071 (2013.01); C12N 9/93 (2013.01); C12N 15/8247 (2013.01); C12Y 114/19 (2013.01); C12Y 114/19003 (2013.01); C12Y 114/19006 (2013.01); C12Y 602/01003 (2013.01)] 10 Claims
 
1. A method for increasing total tocopherol content of a plant relative to a control plant, comprising:
(a) providing a transgenic plant transformed with multiple expression cassettes comprising transgenes which comprise at least one polynucleotide encoding a delta-12-desaturase, at least one polynucleotide encoding a delta-6-desaturase, at least one polynucleotide encoding a delta-6-elongase, and at least one polynucleotide encoding a delta-5-desaturase; and
(b) obtaining transgenic seed oil from the transgenic plant, wherein said transgenic seed oil comprises increased n-3 very long chain polyunsaturated fatty acid (VLC-PUFA) content as compared to a control plant of the same species, wherein the increased n-3 VLC-PUFA content comprises EPA, DPA, and/or DHA; and
(c) measuring total tocopherol content in said transgenic seed oil, wherein said transgenic seed oil has increased total tocopherol content as compared to a control plant of the same species, wherein the transgenic plant is an oilseed plant of the genus Brassica, and wherein expression of said transgenes in said transgenic plant results in said increase in total tocopherol content in said transgenic seed oil.