US 11,771,065 B2
Formulations and methods for promoting honeybee health
Justin Jay Brown, Atwater, CA (US)
Assigned to API HOLDINGS, LLC, Merced, CA (US)
Appl. No. 15/753,899
Filed by API HOLDINGS, LLC, Merced, CA (US)
PCT Filed Aug. 18, 2016, PCT No. PCT/US2016/047645
§ 371(c)(1), (2) Date Feb. 20, 2018,
PCT Pub. No. WO2017/031371, PCT Pub. Date Feb. 23, 2017.
Claims priority of provisional application 62/207,523, filed on Aug. 20, 2015.
Prior Publication US 2018/0235191 A1, Aug. 23, 2018
Int. Cl. A01K 51/00 (2006.01); A01N 63/22 (2020.01); A61K 31/538 (2006.01); A61K 35/742 (2015.01); A61K 47/02 (2006.01); A61K 47/12 (2006.01)
CPC A01K 51/00 (2013.01) [A01N 63/22 (2020.01); A61K 31/538 (2013.01); A61K 35/742 (2013.01); A61K 47/02 (2013.01); A61K 47/12 (2013.01)] 14 Claims
 
1. A formulation for promoting honeybee health, the formulation comprising:
an aqueous solution comprising an organic acid, wherein the organic acid comprises humic acid at 0.1 to 10 Kg humic acid per 10 liters total volume;
one or more buffering agents, wherein the one or more buffering agents are citric acid and sodium bicarbonate;
one or more coloring dyes;
Bacillus subtilis;
Bacillus pumilus;
Bacillus licheniformis;
Bacillus megaterium; and
Bacillus laterosporus,
wherein the formulation further comprises nitrogen, phosphate, potassium, calcium, and one or more amino acids selected from the group consisting of alanine, arginine, aspartic acid, glutamic acid, glycine, histidine, isoleucine, lysine, phenylalanine, proline, serine, threonine, valine, and tyrosine, and
wherein promoting honeybee health comprises increasing resistance to mite infestation and reducing levels of Nosema infection.