US 11,771,028 B2
Use of green microalgae to improve plant growth
Robert Baard, Amsterdam (NL); Wishwas Abhyankar, Amsterdam (NL); and Stanley Brul, Amsterdam (NL)
Assigned to ALGAE INNOVATIONS NETHERLANDS B.V., Amsterdam (NL); and UNIVERSITEIT VAN AMSTERDAM, Amsterdam (NL)
Appl. No. 17/253,816
Filed by Algae Innovations Netherlands B.V., Amsterdam (NL); and Universiteit van Amsterdam, Amsterdam (NL)
PCT Filed Jun. 21, 2019, PCT No. PCT/EP2019/066498
§ 371(c)(1), (2) Date Dec. 18, 2020,
PCT Pub. No. WO2019/243587, PCT Pub. Date Dec. 26, 2019.
Claims priority of application No. 18179068 (EP), filed on Jun. 21, 2018.
Prior Publication US 2021/0171413 A1, Jun. 10, 2021
Int. Cl. A01G 33/00 (2006.01); C05G 5/23 (2020.01); C05F 11/00 (2006.01); C05G 5/27 (2020.01); C05G 3/00 (2020.01)
CPC A01G 33/00 (2013.01) [C05F 11/00 (2013.01); C05G 5/23 (2020.02); C05G 5/27 (2020.02); C05G 3/00 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method for preparing a liquid composition comprising living green microalgae to improve plant growth, wherein the method comprises:
culturing green microalgae in an open pond in an aqueous medium at pH 10 to pH 12, the medium comprising less than 5 mM sodium, and supplying hydrogen carbonate as the inorganic carbon source;
harvesting the green microalgae by sedimentation or centrifugation to obtain a liquid composition comprising living green microalgae, the liquid composition having a dry matter content in the range of 0.5% w/w to 10% w/w and a sodium content of less than 5 mM.
 
8. A method for promoting plant growth, comprising applying the liquid composition obtained by the method of claim 1 to a plant to promote growth of the plant.
 
9. A liquid composition comprising living green microalgae, maximally 5 mM sodium and a dry matter content in the range of 0.5% w/w to 10% w/w.
 
12. A method for improving growth, nutrient assimilation, efficiency of metabolism or photosynthesis of a plant, seed, tuber or bulb, comprising applying the liquid composition of claim 9 to a plant, seed, tuber or bulb to improve growth, nutrient assimilation, efficiency of metabolism or photosynthesis.