US 12,428,670 B2
Methods and systems for analyzing nucleic acid molecules
Andrew Kennedy, San Diego, CA (US); Stefanie Ann Ward Mortimer, Morgan Hill, CA (US); Helmy Eltoukhy, Atherton, CA (US); AmirAli Talasaz, Atherton, CA (US); and Diana Abdueva, Orinda, CA (US)
Assigned to Guardant Health, Inc., Palo Alto, CA (US)
Filed by GUARDANT HEALTH, INC., Palo Alto, CA (US)
Filed on Apr. 3, 2024, as Appl. No. 18/625,882.
Application 18/625,882 is a continuation of application No. 18/061,898, filed on Dec. 5, 2022, granted, now 11,952,616.
Application 18/061,898 is a continuation of application No. 16/450,918, filed on Jun. 24, 2019, granted, now 11,519,019, issued on Dec. 6, 2022.
Application 16/450,918 is a continuation of application No. PCT/US2017/068329, filed on Dec. 22, 2017.
Claims priority of provisional application 62/550,540, filed on Aug. 25, 2017.
Claims priority of provisional application 62/512,936, filed on May 31, 2017.
Claims priority of provisional application 62/438,240, filed on Dec. 22, 2016.
Prior Publication US 2024/0279714 A1, Aug. 22, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6874 (2018.01); C12Q 1/6886 (2018.01)
CPC C12Q 1/6806 (2013.01) [C12N 15/1065 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6886 (2013.01); C12Q 2600/154 (2013.01)] 23 Claims
 
1. A method of analyzing nucleic acids for detecting cancer recurrence, comprising:
(a) purifying cell-free nucleic acids from a bodily fluid sample obtained from a subject;
(b) physically fractionating the cell-free nucleic acids to generate two or more partitions, wherein the physical fractionating comprises fractionating nucleic acids based on one or more characteristics, wherein the one or more characteristics comprises methylation status;
(c) sequencing at least a portion of nucleic acids from each of the two or more partitions at a sequence read depth of at least about five thousand sequence reads per base to generate a set of sequencing reads; and
(d) analyzing the set of sequence reads to detect the one or more characteristics.