US 12,428,639 B2
Plant DNA methyltransferases and uses thereof
Assaf Zemach, Tel-Aviv (IL); Nir Ohad, Tel-Aviv (IL); Rafael Yaari, Tel-Aviv (IL); Aviva Katz, Tel-Aviv (IL); and Katherine Domb, Tel-Aviv (IL)
Assigned to Ramot at Tel-Aviv University Ltd., Tel-Aviv (IL)
Appl. No. 17/436,167
Filed by Ramot at Tel-Aviv University Ltd., Tel-Aviv (IL)
PCT Filed Mar. 4, 2020, PCT No. PCT/IL2020/050254
§ 371(c)(1), (2) Date Sep. 3, 2021,
PCT Pub. No. WO2020/178831, PCT Pub. Date Sep. 10, 2020.
Claims priority of provisional application 62/813,805, filed on Mar. 5, 2019.
Prior Publication US 2022/0135969 A1, May 5, 2022
Int. Cl. C12N 15/11 (2006.01); A01H 11/00 (2006.01); C12N 9/10 (2006.01); C12N 9/22 (2006.01)
CPC C12N 15/111 (2013.01) [A01H 11/00 (2013.01); C12N 9/1007 (2013.01); C12N 9/22 (2013.01); C12N 2310/20 (2017.05); C12Y 201/01037 (2013.01)] 8 Claims
 
1. A mammalian cell comprising an expression vector which comprises a polynucleotide encoding a fusion protein, said fusion protein comprising a DNA targeting moiety linked to a catalytic domain of a plant DNA methyltransferase 3 (DNMT3) protein, wherein the cell comprises a gene and a regulatory sequence thereof, that has a higher methylation status at a CHH site than a methylation status at said CHH site of said gene in the absence of the fusion protein, wherein said DNMT3 is not a domain rearranged methyltransferase (DRM).