US 12,427,117 B2
Lipid nanoparticles manufacturing chip, lipid nanoparticles manufacturing system having the same, and lipid nanoparticles manufacturing method
Euidon Han, Gyeonggi-do (KR); Chan Hee Chon, Gyeonggi-do (KR); Dong Hoon Kim, Gyeonggi-do (KR); and Ju Hee Kim, Gyeonggi-do (KR)
Assigned to INVENTAGE LAB INC., Gyeonggi-Do (KR)
Appl. No. 18/024,743
Filed by Inventage Lab Inc., Gyeonggi-do (KR)
PCT Filed May 11, 2022, PCT No. PCT/KR2022/006749
§ 371(c)(1), (2) Date Mar. 3, 2023,
PCT Pub. No. WO2022/240193, PCT Pub. Date Nov. 17, 2022.
Claims priority of application No. 10-2021-0060963 (KR), filed on May 11, 2021; and application No. 10-2022-0057190 (KR), filed on May 10, 2022.
Prior Publication US 2023/0285312 A1, Sep. 14, 2023
Int. Cl. A61K 9/51 (2006.01); B01F 23/40 (2022.01); B01F 23/41 (2022.01); B01F 23/451 (2022.01); B01F 23/80 (2022.01); B01F 33/301 (2022.01); B01L 3/00 (2006.01); C12N 15/88 (2006.01); B01F 101/22 (2022.01)
CPC A61K 9/5192 (2013.01) [A61K 9/5123 (2013.01); B01F 23/4143 (2022.01); B01F 23/4145 (2022.01); B01F 23/451 (2022.01); B01F 23/483 (2022.01); B01F 23/49 (2022.01); B01F 23/808 (2022.01); B01F 33/3017 (2022.01); B01L 3/502753 (2013.01); C12N 15/88 (2013.01); B01F 2101/22 (2022.01); B01L 2200/16 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0883 (2013.01)] 12 Claims
 
1. A lipid nanoparticles manufacturing chip comprising:
a mixer unit for forming a mixed solution by mixing a first raw material containing an active ingredient and a second raw material containing a lipid;
a dilution unit that is connected to the mixer unit and dilutes the mixed solution using a diluent solution to make a diluted mixed solution; and
a concentration unit connected to the dilution unit and for obtaining a concentrated solution by concentrating lipid nanoparticles (LNP) from the diluted mixed solution,
wherein the mixer unit, the dilution unit and the concentration unit are formed on one substrate; and
the concentration unit comprises:
a main flow path connected to the dilution unit, wherein the diluted mixed solution flows in one direction;
an ion exchange channel in contact with the main flow path;
a buffer solution channel spaced apart from the main flow path and in contact with the ion exchange channel, wherein a voltage is applied to a buffer solution of the buffer solution channel to form an ion enrichment zone and an ion depletion zone in a solution within the main flow path, wherein the diluted mixed solution is separated into the concentrated solution and a solution other than the concentrated solution on the main flow path by the ion exchange channel and the buffer solution channel to which a voltage is applied;
wherein, at a position after the diluted mixed solution on the main flow path is passed through the ion exchange channel and separated into the concentrated solution and the solution other than the concentrated solution, the main flow path is divided, into:
a lipid nanoparticle acquisition flow path connected downstream to the mainflow path and configured for obtaining the concentrated solution flowing in the one direction; and
a collecting flow path connected downstream to the main flow path and configured for collecting the solution other than the concentrated solution flowing in the one direction,
wherein the concentrated solution and the solution other than the concentrated solution are simultaneously and continuously obtained and collected.