US 12,097,302 B2
Method for producing a cultured cell sheet for tissue regeneration
Masato Sato, Isehara (JP); Chikako Sato, Tokyo (JP); Tomomi Kotoku, Tokyo (JP); and Tomoko Kawake, Tokyo (JP)
Assigned to CELLSEED INC., Tokyo (JP); and TOKAI UNIVERSITY EDUCATIONAL SYSTEM, Tokyo (JP)
Filed by CellSeed Inc., Tokyo (JP); and TOKAI UNIVERSITY EDUCATIONAL SYSTEM, Tokyo (JP)
Filed on Apr. 26, 2022, as Appl. No. 17/729,608.
Application 17/729,608 is a division of application No. 15/555,846, abandoned, previously published as PCT/JP2017/031136, filed on Aug. 30, 2017.
Claims priority of application No. 2017-033924 (JP), filed on Feb. 24, 2017.
Prior Publication US 2022/0257833 A1, Aug. 18, 2022
Int. Cl. C12N 5/077 (2010.01); A61L 27/36 (2006.01); A61L 27/38 (2006.01); C12N 5/0775 (2010.01)
CPC A61L 27/3612 (2013.01) [A61L 27/3817 (2013.01); A61L 27/3834 (2013.01); A61L 27/3852 (2013.01); C12N 5/0655 (2013.01); C12N 5/0668 (2013.01); A61L 2430/06 (2013.01); C12N 2533/30 (2013.01)] 19 Claims
 
1. A method for producing a cell sheet for cartilage repair, the cell sheet being formed from a culture of cells derived from a cartilage tissue, the method comprising:
culturing cells derived from a cartilage tissue on a surface of a membrane, a temperature-responsive polymer being immobilized on the surface, to produce a cell sheet of cultured cells, and
evaluating the cell sheet of cultured cells as a cell sheet suitable for cartilage repair, when the cell sheet of the cultured cells shows positive in immunostaining using an antibody against type 1 collagen and negative in immunostaining using an antibody against type 2 collagen,
wherein the culturing is stopped before the cell sheet of cultured cells evaluated as the cell sheet suitable for cartilage repair becomes positive for immunostaining using an antibody against type II collagen.