	US 11,767,374 B2
	*Method and use of Radix puerariae* polysaccharide in promoting lipid-lowering activity**
Hui Ouyang, Nanchang (CN); Quan Wen, Nanchang (CN); WeiFeng Zhu, Nanchang (CN); YuLin Feng, Nanchang (CN); RongHua Liu, Nanchang (CN); YongMei Guan, Nanchang (CN); Kai Qian, Nanchang (CN); and Hui Du, Nanchang (CN)
Assigned to JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE, Nanchang (CN)
Appl. No. 17/597,536
Filed by JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE, Nanchang (CN)
PCT Filed Mar. 8, 2021, PCT No. PCT/CN2021/079531 § 371(c)(1), (2) Date Jan. 10, 2022, PCT Pub. No. WO2021/180031, PCT Pub. Date Sep. 16, 2021.
Claims priority of application No. 202010156044.9 (CN), filed on Mar. 9, 2020.
Prior Publication US 2022/0259331 A1, Aug. 18, 2022
Int. Cl. C08B 37/00 (2006.01); A61P 3/06 (2006.01); A61K 31/716 (2006.01); A61K 31/715 (2006.01); A61K 36/488 (2006.01)

CPC C08B 37/0003 (2013.01) [A61K 31/715 (2013.01); A61K 31/716 (2013.01); A61K 36/488 (2013.01); A61P 3/06 (2018.01); C08B 37/0006 (2013.01); C08B 37/0009 (2013.01)]

2 Claims

1. A method of using Radix Puerariae polysaccharide QL to promote lipid-lowering activity in a subject, comprising:

preparing a therapeutically effective dosage of a Radix Puerariae polysaccharide QL;

wherein, the Radix Puerariae polysaccharide QL is a homopolysaccharide composed of one monosaccharide glucose, and has a molecular weight of 10 KDa to 60 KDa, a protein content of 0.64%, no glycuronic acid, and a sugar content of 98.7%;

wherein, an infrared spectrum (IR) of the Radix Puerariae polysaccharide QL shows typical polysaccharide absorption peaks comprising a hydroxyl absorption peak around 3,300 cm⁻¹, and a glycosyl absorption peak around 957 cm⁻¹, but shows no carbonyl absorption peak at 1,700 cm⁻¹, the IR of the Radix Puerariae polysaccharide QL is consistent with a measured glycuronic acid content;

wherein, gas chromatography-mass spectrometry (GC-MS) analysis confirms that the Radix Puerariae polysaccharide QL is composed of glucose, wherein most glucose units are linked through α-1,3-Glu and a small number of glucose units are linked through terminal-Glu; and wherein, an atomic force microscopy (AFM) test shows that a spatial structure of the Radix Puerariae polysaccharide QL has a spherical characteristic; administering the therapeutically effective dosage of a Radix Puerariae polysaccharide QL in vivo to the subject at a dosage between 25 mg/kg and 100 mg/kg.