US 11,767,348 B2
OmpG variants
Cynthia Cech, Mountain View, CA (US); Tim Craig, Mountain View, CA (US); Christos Tzitzilonis, Mountain View, CA (US); Alexander Yang, Mountain View, CA (US); Liv Jensen, Mountain View, CA (US); Charlotte Yang, Mountain View, CA (US); Corissa Harris, Mountain View, CA (US); Matthew Dipietro, Gilroy, CA (US); and Dhruti Dalal, Sunnyvale, CA (US)
Assigned to Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed by Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed on Jul. 10, 2020, as Appl. No. 16/925,848.
Application 16/925,848 is a continuation of application No. 15/762,092, granted, now 10,752,658, previously published as PCT/EP2016/072224, filed on Sep. 20, 2016.
Claims priority of provisional application 62/222,197, filed on Sep. 22, 2015.
Claims priority of provisional application 62/333,672, filed on May 9, 2016.
Prior Publication US 2020/0392191 A1, Dec. 17, 2020
Int. Cl. A61K 39/02 (2006.01); C07K 14/245 (2006.01); C12Q 1/6874 (2018.01); C12Q 1/68 (2018.01); G01N 33/68 (2006.01); C12N 9/12 (2006.01)
CPC C07K 14/245 (2013.01) [C12N 9/1252 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6874 (2013.01); C12Y 207/07007 (2013.01); G01N 33/6803 (2013.01); C07K 2319/00 (2013.01)] 24 Claims
 
1. A circular permutation variant of a parental outer membrane protein G (OmpG) polypeptide, the variant comprising a rearranged parental OmpG polypeptide in which a portion of the C-terminal end of the parental OmpG polypeptide comprising a beta strand is moved to the N-terminal end of the OmpG polypeptide,
wherein the parental OmpG polypeptide from which the variant is derived comprises (i) an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth as SEQ ID NO: 2 and (ii) a deletion of at least one loop-6 amino acid and
wherein the circular permutation variant retains the ability to form a nanopore.