CPC C07K 14/245 (2013.01) [C12N 9/1252 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6874 (2013.01); C12Y 207/07007 (2013.01); G01N 33/6803 (2013.01); C07K 2319/00 (2013.01)] | 24 Claims |
1. A circular permutation variant of a parental outer membrane protein G (OmpG) polypeptide, the variant comprising a rearranged parental OmpG polypeptide in which a portion of the C-terminal end of the parental OmpG polypeptide comprising a beta strand is moved to the N-terminal end of the OmpG polypeptide,
wherein the parental OmpG polypeptide from which the variant is derived comprises (i) an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth as SEQ ID NO: 2 and (ii) a deletion of at least one loop-6 amino acid and
wherein the circular permutation variant retains the ability to form a nanopore.
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