	US 11,766,488 B2
	Compositions and methods comprising improvements of CRISPR guide RNAS using the H1 promoter
Vinod Jaskula-Ranga, Cambridge, MA (US); Donald Zack, Baltimore, MD (US); and Derek Welsbie, Baltimore, MD (US)
Assigned to The Johns Hopkins University, Baltimore, MD (US)
Appl. No. 16/315,458
Filed by The Johns Hopkins University, Baltimore, MD (US)
PCT Filed Jul. 5, 2017, PCT No. PCT/US2017/040707 § 371(c)(1), (2) Date Jan. 4, 2019, PCT Pub. No. WO2018/009534, PCT Pub. Date Jan. 11, 2018.
Claims priority of provisional application 62/358,335, filed on Jul. 5, 2016.
Prior Publication US 2019/0314521 A1, Oct. 17, 2019
Int. Cl. A61K 48/00 (2006.01); A61K 9/00 (2006.01); C12N 15/86 (2006.01)

CPC A61K 48/005 (2013.01) [A61K 9/0014 (2013.01); A61K 9/0019 (2013.01); C12N 15/86 (2013.01); C12N 2310/20 (2017.05); C12N 2330/51 (2013.01); C12N 2710/10344 (2013.01); C12N 2750/14143 (2013.01)]

19 Claims

1. A non-naturally occurring CRISPR system comprising one or more vectors comprising: a bidirectional 7sk promoter, wherein

a) one side of the bidirectional 7sk promoter is operably linked to at least one nucleotide sequence encoding a CRISPR guide RNA (gRNA), wherein the gRNA hybridizes with a target sequence of a DNA molecule or RNA molecule in a cell; and)

b) the other side of the bidirectional 7sk promoter is operably linked to a nucleotide sequence encoding a CRISPR enzyme,

wherein the gRNA targets and hybridizes with the target sequence and the CRISPR enzyme cleaves one or both strands of the DNA molecule or RNA molecule.