US 11,766,465 B2
External composition for wound healing containing lactobacillus fermentation product and method for promoting wound healing using the same
Hsing-Chun Kuo, Taoyuan (TW); Chin-Chu Chen, Taoyuan (TW); Yen-Lien Chen, Taoyuan (TW); Shih-Wei Lin, Taoyuan (TW); Yen-Po Chen, Taoyuan (TW); Ci-Sian Wang, Taoyuan (TW); Yu-Hsin Hou, Taoyuan (TW); Yang-Tzu Shih, Taoyuan (TW); Ching-Wen Lin, Taoyuan (TW); Ya-Jyun Chen, Taoyuan (TW); Jia-Lin Jiang, Taoyuan (TW); You-Shan Tsai, Taoyuan (TW); and Zi-He Wu, Taoyuan (TW)
Assigned to GRAPE KING BIO LTD, Taoyuan (TW)
Filed by GRAPE KING BIO LTD, Taoyuan (TW)
Filed on Oct. 29, 2021, as Appl. No. 17/514,670.
Claims priority of application No. 109139743 (TW), filed on Nov. 13, 2020.
Prior Publication US 2022/0152129 A1, May 19, 2022
Int. Cl. A61K 35/747 (2015.01); A61P 17/02 (2006.01); A61K 9/00 (2006.01)
CPC A61K 35/747 (2013.01) [A61K 9/0014 (2013.01); A61P 17/02 (2018.01)] 5 Claims
 
1. A method for promoting wound healing using an external composition, comprising:
administering a composition comprising a Lactobacillus fermentation product as an effective component onto a skin of a subject in need thereof, wherein the Lactobacillus fermentation product is a bacteria-free concentrated filtrate from a fermentation step, a solid-liquid separation step and a concentration step of a fermentation substrate by Lactobacillus, the concentrated filtrate is concentrated to 5% to 20% of an initial volume of the fermentation substrate, the Lactobacillus is Lactobacillus plantarum GKM3 (deposited in China General Microbiological Culture Collection Center (CGMCC) with a deposition number of CGMCC 14565 on Aug. 25, 2017, and in Bioresource Collection and Research Center (BCRC) of Food Industry Research and Development Institute of the Republic of China, No. 331 Food Road, Hsinchu, Taiwan with deposit date of Jul. 14, 2017 and deposit number of BCRC 910787), and the fermentation substrate is selected from a group consisting of MRS agar, PCA agar, M17 agar, Rogosa agar, TOS-MUP agar, and any combination thereof; and
a pharmaceutically acceptable absorbent carrier or carrying agent for loading the effective component.