	US 11,766,457 B2
	Immunosuppressive antigen-specific chimeric antigen receptor Treg cells for prevention and/or treatment of autoimmune and alloimmune disorders
Juan C. Jaume, Toledo, OH (US); and Shahnawaz Imam, Toledo, OH (US)
Assigned to The University of Toledo, Toledo, OH (US)
Filed by The University of Toledo, Toledo, OH (US)
Filed on May 14, 2021, as Appl. No. 17/320,663.
Prior Publication US 2022/0362294 A1, Nov. 17, 2022
Int. Cl. A61K 35/17 (2015.01); C07K 14/725 (2006.01); C07K 14/705 (2006.01)

CPC A61K 35/17 (2013.01) [C07K 14/7051 (2013.01); C07K 14/70521 (2013.01); C07K 2319/02 (2013.01); C07K 2319/03 (2013.01); C07K 2319/33 (2013.01)]

10 Claims

1. An immunoresponsive cell comprising:

a chimeric antigen receptor (CAR) that binds to glutamic acid decarboxylase 65 kDA (GAD65) in the cell;

the CAR comprising:

a) an intracellular signaling domain of a CD3ζ polypeptide and an intracellular signaling domain of CD28 hinge-transmembrane-intracellular region, and

b) an extracellular polypeptide comprising an amino acid sequence that is a GAD65 MAb antigen binding domain;

wherein the extracellular polypeptide comprises SEQ ID NO: 7, or an amino acid sequence having at least 95% identity to SEQ ID NO: 7.