| CPC G16H 50/20 (2018.01) [G06V 10/774 (2022.01); G06V 10/945 (2022.01); G06V 20/695 (2022.01); G06V 20/698 (2022.01); G06V 20/70 (2022.01); G06V 2201/03 (2022.01)] | 19 Claims |

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13. A system comprising:
one or more data processors; and
a non-transitory computer readable storage medium containing instructions which, when executed on the one or more data processors, cause the one or more data processors to perform a set of operations including:
accessing a digital pathology image that depicts a tissue slice stained with multiple stains, each of the multiple stains staining for a corresponding biomarker of a set of biomarkers, wherein the multiple stains include at least three stains;
generating, using a first machine-learning model, a segmented image that identifies at least:
a predicted diseased region in the digital pathology image; and
a background region in the digital pathology image, wherein the background region indicates that signals that are present within the background region are not to be assessed when analyzing signals of the set of biomarkers;
detecting depictions of a set of cells in the digital pathology image;
generating, using a second machine-learning model, a cell classification for each cell of the set of cells, wherein the cell classification is selected from a set of potential classifications that indicate which, if any, of the set of biomarkers are expressed in the cell;
detecting that a subset of the set of cells in the digital pathology image are within the background region; and
in response to detecting that the subset of the set of cells in the digital pathology image are within the background region, updating the cell classification for each cell of at least some cells in the subset to be a background classification that was not included in the set of potential classifications.
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