| CPC G02B 21/244 (2013.01) [G02B 21/008 (2013.01); G02B 21/367 (2013.01); G06T 7/0014 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/10148 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30168 (2013.01)] | 20 Claims |

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1. A method for operating a microscope scanner comprising a detector array, the method comprising:
performing a pre-scan so as to generate a pre-scan image of a target, wherein the target comprises a sample, and wherein a depth of focus for the pre-scan is in excess of 500 micrometres;
performing a first imaging scan of one or more area(s) of interest, AOI, on the target, wherein performing the first imaging scan comprises:
moving the detector array relative to the target along an image scan path and acquiring an image of the target at each of a plurality of locations along the image scan path using the detector array, and wherein a depth of focus for each image acquired along the image scan path is below 3 micrometres;
generating focus control data during the first imaging scan, wherein generating focus control data comprises calculating a focus merit value at each said location along the image scan path; and
adjusting a focal height of the detector array along the image scan path based on said focus merit values; and
analysing an efficacy of the first imaging scan using the focus control data and determining a change to one or more scanning parameters from the first imaging scan, for the performance of a second imaging scan, based on this analysis.
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