US 12,422,657 B2
Apparatus and method for detecting a spatial elongation of at least one adherent biological cell
Tobias Neckernuss, Eislingen (DE); Daniel Geiger, Mittelbiberach (DE); and Othmar Marti, Ulm (DE)
Assigned to Universität Ulm, Ulm (DE)
Appl. No. 17/270,014
Filed by UNIVERSITÄT ULM, Ulm (DE)
PCT Filed Aug. 20, 2019, PCT No. PCT/EP2019/072212
§ 371(c)(1), (2) Date Sep. 14, 2021,
PCT Pub. No. WO2020/038914, PCT Pub. Date Feb. 27, 2020.
Claims priority of application No. 10 2018 213 965.2 (DE), filed on Aug. 20, 2018.
Prior Publication US 2022/0214331 A1, Jul. 7, 2022
Int. Cl. G02B 21/06 (2006.01); C12M 1/42 (2006.01); G01N 15/14 (2024.01)
CPC G02B 21/06 (2013.01) [G01N 15/14 (2013.01); C12M 35/02 (2013.01); G01N 2015/1495 (2013.01)] 17 Claims
OG exemplary drawing
 
1. An apparatus for detecting a spatial elongation of at least one adherent cell, comprising
(a) at least one biological cell on a substrate;
(b) a laser for irradiating the at least one biological cell with electromagnetic radiation in a direction of irradiation, wherein the laser is configured to irradiate electromagnetic radiation on the at least one biological cell that effects a spatial elongation of the at least one biological cell in a direction parallel to the direction of irradiation; and
(c) a detector for detecting a spatial elongation of the at least one biological cell in a direction parallel to the direction of irradiation;
wherein the at least one biological cell is a biological cell adherent on the substrate, and
wherein the laser is configured to irradiate the electromagnetic radiation at a power in the range from 50 to 2000 mW,
wherein the apparatus has a processing unit that is configured to receive and evaluate data of the detector and/or of a data storage of the apparatus, and
wherein the processing unit is configured to carry out a conversion of the data on a spatial elongation of the at least one cell in an unknown length into a spatial elongation of the at least one cell in a known length, wherein the processing unit is configured to utilize, for carrying out the conversion, diffraction images detected at the detector and a point spread function.