US 12,422,656 B2
Methods and systems for multidimensional imaging
Hu Cang, San Diego, CA (US); and Eli N. Glezer, Del Mar, CA (US)
Assigned to Singular Genomics Systems, Inc., San Diego, CA (US)
Filed by Singular Genomics Systems, Inc, San Diego, CA (US)
Filed on Jun. 7, 2023, as Appl. No. 18/331,032.
Application 18/331,032 is a continuation of application No. 17/691,731, filed on Mar. 10, 2022, granted, now 11,714,271.
Application 17/691,731 is a continuation of application No. PCT/US2021/050077, filed on Sep. 13, 2021.
Claims priority of provisional application 63/077,852, filed on Sep. 14, 2020.
Prior Publication US 2023/0333361 A1, Oct. 19, 2023
Int. Cl. G02B 21/02 (2006.01); G01N 21/64 (2006.01); G02B 6/04 (2006.01); G02B 21/00 (2006.01); G02B 27/10 (2006.01)
CPC G02B 21/02 (2013.01) [G01N 21/6402 (2013.01); G01N 21/6458 (2013.01); G02B 21/0032 (2013.01); G02B 21/0076 (2013.01); G02B 21/008 (2013.01); G02B 27/106 (2013.01); G01N 2021/6463 (2013.01); G01N 2021/6484 (2013.01); G02B 6/04 (2013.01); G02B 21/0036 (2013.01)] 30 Claims
OG exemplary drawing
 
1. An imaging system, comprising:
a first bundle of optic fibers providing a first pair of excitation beams and a second bundle of optic fibers providing a second pair of excitation beams, wherein the first bundle and the second bundle are spatially separated by a first offset distance;
at least a first light source and a second light source;
at least a first splitter coupled to the first light source and a second splitter coupled to the second light source, wherein the first splitter splits light of the first light source into the first bundle of optic fibers and the second splitter splits light of the second light sources into the second bundle of optic fibers, wherein each of the first bundle and the second bundle includes at least one optic fiber from the first light source and at least one optic fiber from the second light source;
an objective lens, wherein the first pair of excitation beams are focused into a sample at a first focus depth along an optical axis; and wherein the second pair of excitation beams are focused into the sample at a second focus depth along the optical axis; wherein the first focus depth is separated from the second focus depth by a second offset distance; and
an image sensor, wherein at least a portion of the image sensor is oriented at an oblique orientation relative to the optical axis.