US 12,422,446 B2
Pretreatment method of an automatic analyzer
Makoto Nogami, Tokyo (JP); Shinya Matsuoka, Tokyo (JP); Daisuke Ebihara, Tokyo (JP); and Yuichiro Hashimoto, Tokyo (JP)
Assigned to HITACHI HIGH-TECH CORPORATION, Tokyo (JP)
Appl. No. 17/601,469
Filed by HITACHI HIGH-TECH CORPORATION, Tokyo (JP)
PCT Filed Mar. 9, 2020, PCT No. PCT/JP2020/009996
§ 371(c)(1), (2) Date Oct. 5, 2021,
PCT Pub. No. WO2020/225971, PCT Pub. Date Nov. 12, 2020.
Claims priority of application No. 2019-088019 (JP), filed on May 8, 2019.
Prior Publication US 2022/0276270 A1, Sep. 1, 2022
Int. Cl. G01N 35/00 (2006.01); G01N 35/04 (2006.01)
CPC G01N 35/0098 (2013.01) [G01N 2035/00356 (2013.01); G01N 2035/00534 (2013.01); G01N 2035/0446 (2013.01)] 5 Claims
 
1. A method of pretreating a sample for an automatic analyzer comprising the steps of:
in one assay protocol, which includes a plurality of cycles in a predetermined time:
adding first magnetic beads to the sample, which contains a plurality of substances to be measured, the first magnetic beads each having surfaces modified with an antibody as a functional group that specifically binds to a structure of a first substance to be measured among the plurality of substances to be measured in the sample;
adding second magnetic beads to the sample, the second magnetic beads each having surfaces modified with an octadecylsilyl group that specifically binds to a second substance to be measured among the plurality of substances to be measured in the sample;
extracting the first magnetic beads and the second magnetic beads from the sample; and
separating, by a first eluate, the first substance to be measured from the first magnetic beads;
transferring the first eluate to a mass spectrometer; and
after transferring the first eluate to the mass spectrometer, separating, by a second eluate, the second substance to be measured from the second magnetic beads,
wherein each step is performed in one or more of the cycles of the one assay protocol.