US 12,422,438 B2
Means and methods for single molecule peptide sequencing
Nico Callewaert, Nevele (BE); Sven Eyckerman, Nazareth (BE); and Simon Devos, Sint-Michiels (BE)
Assigned to VIB VZW, Ghent (BE); and Universiteit Gent, Ghent (BE)
Filed by VIB VZW, Ghent (BE); and Universiteit Gent, Ghent (BE)
Filed on May 14, 2024, as Appl. No. 18/663,402.
Application 18/663,402 is a continuation of application No. 17/817,908, filed on Aug. 5, 2022, granted, now 12,019,076, issued on Jun. 25, 2024.
Application 17/817,908 is a continuation of application No. 16/650,230, granted, now 11,573,238, issued on Feb. 7, 2023, previously published as PCT/EP2018/076532, filed on Sep. 28, 2018.
Claims priority of application No. 1715684 (GB), filed on Sep. 28, 2017.
Prior Publication US 2025/0076303 A1, Mar. 6, 2025
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); C12N 9/48 (2006.01); C12N 9/64 (2006.01)
CPC G01N 33/6812 (2013.01) [C12N 9/485 (2013.01); C12N 9/641 (2013.01); G01N 33/68 (2013.01); G01N 33/6818 (2013.01); G01N 33/6824 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method of detecting terminal amino acid cleavage, the method comprising:
a) contacting an immobilized peptide with one or more peptide-binding agents;
b) measuring residence times of the one or more peptide-binding agents on the immobilized peptide, wherein each residence time corresponds to a single molecule binding event between a peptide-binding agent and a terminal amino acid of the immobilized peptide; and
c) detecting one or more changes in the measured residence times, wherein a change in the measured residence times is indicative of terminal amino acid cleavage from the immobilized peptide.