US 12,422,431 B2
Capture flow assay device and methods
Robert Marks, Omer (IL); Tim Axelrod, Haniel (IL); and Ariel Kushmaro, Beer Yaakov (IL)
Assigned to B. G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY, Beer Sheva (IL)
Appl. No. 17/419,865
Filed by B. G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY, Beer Sheva (IL)
PCT Filed Dec. 31, 2019, PCT No. PCT/IL2019/051446
§ 371(c)(1), (2) Date Jun. 30, 2021,
PCT Pub. No. WO2020/141525, PCT Pub. Date Jul. 9, 2020.
Claims priority of provisional application 62/786,611, filed on Dec. 31, 2018.
Prior Publication US 2022/0082560 A1, Mar. 17, 2022
Int. Cl. G01N 33/543 (2006.01); B01L 3/00 (2006.01)
CPC G01N 33/54388 (2021.08) [B01L 3/5023 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/069 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A device for detecting an analyte in a sample, the device comprising a section 1, a section 2, a section 3, a section 4, and a calibration area wherein:
a. said section 2 is coupled to said section 1, said section 2 comprises a surface comprising a recognition molecule having specific affinity to said analyte, wherein said recognition molecule is linked to a reporter molecule, and wherein said reporter molecule generates a trigger whether bound to said analyte or not;
b. said section 3 is coupled to said section 2 and to said section 4, said section 3 comprises a surface functionalized with said same analyte as present in the sample, wherein binding of said reporter molecule to said analyte in section 3 produces a reduced signal indicating an analyte negative sample; and
c. sections 1 to 4 are arranged along a horizontal axis and in fluid communication allowing lateral flow of fluid from said section 1 through said section 2 and said section 3 to section 4, and said section 4 comprises a surface in contact with a substrate molecule generating a signal in response to said reporter molecule.