US 12,422,413 B2
Automated sample handling system for liquid chromatography-mass spectrometry
Daniel Hasle, Seattle, WA (US)
Filed by FIALab Insruments Inc., Seattle, WA (US)
Filed on Dec. 22, 2022, as Appl. No. 18/086,709.
Prior Publication US 2024/0210363 A1, Jun. 27, 2024
Int. Cl. G01N 30/24 (2006.01)
CPC G01N 30/24 (2013.01) 16 Claims
OG exemplary drawing
 
1. An automated fluid handling system (100) for handling a biological fluid sample prior to being delivered for liquid chromatography-mass spectrometry (LCMS) analysis, comprising:
at least one inlet tube (104) connected to a biological fluid sample source (110);
a first multi-position selector valve (120) fluidly connected to a hollow membrane fiber filter (140); wherein the first multi-position selector valve (120) comprises at least one inlet port (121) for fluidic connection with the inlet tube (104), a waste port (123) and a plug (125);
a second multi-position selector valve (130) fluidly connected to a hollow membrane fiber filter (140), wherein the second multi-position selector valve (130) comprises at least one outlet port (131), a waste port (133), and a plug (135);
a plurality of syringe pumps (150,160) fluidly connected to a hollow membrane fiber filter (140); wherein each of the plurality of syringe pumps (150, 160) is configured to aspire and dispense a fluid;
at least one purification column (170) fluidly connected to an outlet port (131) of the second multi-position selector valve (120);
a titer detection system (180) fluidly connected to the purification column (170), wherein the titer detection system (180) is configured to determine titer concentration value of the biological fluid sample; and
a pre-treatment unit (190) to recapture the measured biological fluid sample coming from the titer detection system (180); wherein the pre-treatment unit (190) is configured to conduct chemical pre-treatment on the measured biological on the biological sample fluid prior to liquid chromatography-mass spectroscopy analysis.