| CPC G01N 29/022 (2013.01) [G01N 29/036 (2013.01); G01N 29/2437 (2013.01); H03H 9/02559 (2013.01); G01N 2291/0423 (2013.01)] | 20 Claims |

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1. A method for harmonic acoustic manipulation of particles, the method comprising:
introducing particles into a fluid positioned over a first surface of a piezoelectric substrate;
generating a first harmonic signal comprising a plurality of harmonic components; and
applying the first harmonic signal to a first harmonic acoustic transducer, wherein the first harmonic acoustic transducer is coupled to the first surface of the piezoelectric substrate and spaced apart from the fluid.
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