US 12,422,367 B2
Methods for determination of virus titer in a sample using Raman spectroscopy
Karin M. Balss, Spring House, PA (US); Zachary Schultz, Columbus, OH (US); and Courtney J. Morder, Johnstown, PA (US)
Assigned to JANSSEN BIOTECH, INC., Horsham, PA (US); and OHIO STATE INNOVATION FOUNDATION, Columbus, OH (US)
Filed by JANSSEN BIOTECH, INC., Horsham, PA (US); and OHIO STATE INNOVATION FOUNDATION, Columbus, OH (US)
Filed on Nov. 21, 2022, as Appl. No. 17/990,887.
Claims priority of provisional application 63/281,441, filed on Nov. 19, 2021.
Prior Publication US 2023/0160829 A1, May 25, 2023
Int. Cl. G01N 21/65 (2006.01); G01J 3/44 (2006.01); G01N 33/569 (2006.01); G06F 17/10 (2006.01)
CPC G01N 21/658 (2013.01) [G01J 3/44 (2013.01); G01N 33/56983 (2013.01); G01N 2201/1293 (2013.01); G06F 17/10 (2013.01)] 26 Claims
OG exemplary drawing
 
1. A method of quantifying viral titer in a sample using surface enhanced Raman spectroscopy (SERS), said method comprising:
providing a sample containing a first virus particle and a second virus particle, wherein the first virus particle differs from the second virus particle by one or more genetic elements;
providing a pre-trained, virus-type specific model for determining viral titer of the first virus particle in the sample;
irradiating the sample with a light source;
acquiring a SERS spectrum of the sample on a gold or silver substrate, wherein the gold or silver substrate does not contain surface-bound reagents that promote virus particle binding; and
quantifying the viral titer of the first virus particle in the sample by applying a virus-specific spectral component of the SERS spectrum associated with the one or more genetic elements of the first virus particle to the model for determining viral titer.