	US 12,422,366 B2
	Non-destructive detection of egg freshness based on Raman spectroscopy
Yunfei Xie, Wuxi (CN); Yuliang Liu, Wuxi (CN); Hang Yu, Wuxi (CN); Weirong Yao, Wuxi (CN); Yahui Guo, Wuxi (CN); Yuliang Cheng, Wuxi (CN); and Yichi Zhang, Wuxi (CN)
Assigned to Jiangnan University, Wuxi (CN)
Filed by Yunfei Xie, Wuxi (CN); Yuliang Liu, Wuxi (CN); Hang Yu, Wuxi (CN); Weirong Yao, Wuxi (CN); Yahui Guo, Wuxi (CN); Yuliang Cheng, Wuxi (CN); and Yichi Zhang, Wuxi (CN)
Filed on Mar. 27, 2021, as Appl. No. 17/214,809.
Application 17/214,809 is a continuation of application No. PCT/CN2020/072891, filed on Jan. 18, 2020.
Claims priority of application No. 202010023268.2 (CN), filed on Jan. 9, 2020.
Prior Publication US 2021/0247318 A1, Aug. 12, 2021
Int. Cl. G01N 21/65 (2006.01); G01N 33/08 (2006.01); G06F 17/18 (2006.01)

CPC G01N 21/65 (2013.01) [G01N 33/08 (2013.01); G06F 17/18 (2013.01)]

4 Claims

1. A method for non-destructive detection of egg freshness, comprising:

(1) acquiring Raman spectral data on surfaces of sample eggs;

(2) measuring values of a physicochemical index for egg freshness of the sample eggs, wherein the physicochemical index for egg freshness is Haugh unit, albumen pH, air chamber diameter or air chamber height;

(3) pretreating the Raman spectral data using selected pretreatment methods, wherein the pretreatment method is first derivative for predicting physicochemical values of air chamber diameter or air chamber height, and wherein the pretreatment method is second derivative for predicting values of Haugh unit or albumen pH,

(4) building a partial least squares regression (PLSR) model using the pretreated Raman spectral data and the measured values of the physicochemical index; and

(5) using the PLSR model and a Raman spectrum of a test egg to obtain a value of the physicochemical index and using the obtained physicochemical index value to detect the freshness of the test egg without breaking the test egg,

wherein parameters for acquiring the Raman spectral data are as follows: excitation wavelength is 785 nm, acquisition waveband is 100-3000 cm⁻¹, acquisition time is 5 s, the acquisition is performed for 3 times, and distance from a probe to the egg surface is 6 mm.