US 12,422,364 B2
Fluorescence spectral shape analysis for analyte recognition
Yun Huang, San Antonio, TX (US); Zhiliang Li, San Antonio, TX (US); Kirk Schanze, Helotes, TX (US); and April Risinger, San Antonio, TX (US)
Assigned to Board of Regents, The University of Texas System, Austin, TX (US)
Appl. No. 16/976,546
Filed by THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM, Austin, TX (US)
PCT Filed Feb. 28, 2019, PCT No. PCT/US2019/020109
§ 371(c)(1), (2) Date Aug. 28, 2020,
PCT Pub. No. WO2019/169169, PCT Pub. Date Sep. 6, 2019.
Claims priority of provisional application 62/672,899, filed on May 17, 2018.
Claims priority of provisional application 62/636,823, filed on Feb. 28, 2018.
Prior Publication US 2021/0003506 A1, Jan. 7, 2021
Int. Cl. G01N 21/64 (2006.01); G01N 33/533 (2006.01); G01N 33/542 (2006.01)
CPC G01N 21/6428 (2013.01) [G01N 33/533 (2013.01); G01N 33/542 (2013.01); G01N 2458/10 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A method of identifying an analyte in a sample comprising:
contacting the analyte, wherein the analyte is a nucleoside, a nucleotide, a nucleotide diphosphate, or a nucleotide triphosphate, with a polycation polymer forming an analyte/polycation polymer complex, wherein the polycation polymer is a P-C-3 polymer having the chemical structure below

OG Complex Work Unit Chemistry
where n is 3 to 1000;
exposing the analyte/polycation polymer complex to one or more excitation wavelengths of light;
detecting an emission spectra at two or more selected wavelengths and a reference wavelength; and
identifying the analyte by analyzing the emission spectra.