US 12,421,626 B2
Cell-stored barcoded deep mutational scanning libraries and uses of the same
Jesse Bloom, Seattle, WA (US); Adam S. Dingens, Seattle, WA (US); Katharine H. D. Crawford, Seattle, WA (US); and Caelan Radford, Seattle, WA (US)
Assigned to Fred Hutchinson Cancer Center, Seattle, WA (US); and University of Washington, Seattle, WA (US)
Appl. No. 17/281,540
Filed by Fred Hutchinson Cancer Center, Seattle, WA (US); and University of Washington, Seattle, WA (US)
PCT Filed Jun. 28, 2019, PCT No. PCT/US2019/039952
§ 371(c)(1), (2) Date Mar. 30, 2021,
PCT Pub. No. WO2020/006494, PCT Pub. Date Jan. 2, 2020.
Claims priority of provisional application 62/692,398, filed on Jun. 29, 2018.
Prior Publication US 2021/0363661 A1, Nov. 25, 2021
Int. Cl. C40B 40/08 (2006.01)
CPC C40B 40/08 (2013.01) 20 Claims
OG exemplary drawing
 
1. A cell-stored barcoded deep mutational scanning library of variants of a viral protein comprising: storage cells, wherein at least 90% of the storage cells comprise a non-self-inactivating viral vector comprising a single homozygous barcoded variant nucleotide sequence encoding a viral protein variant from a set of homozygous barcoded variant nucleotide sequences in the library integrated into the storage cell's genome, wherein the set of homozygous barcoded variant nucleotide sequences collectively encode viral protein variants comprising at least 15 amino acid substitutions at at least 95% of amino acid positions of the viral protein.